To ATCC Valued Customers,

ATCC stands ready to support our customers’ needs during the coronavirus pandemic. If you experience any issues with your products or services, please contact ATCC Customer Service at sales@atcc.org. For Technical questions please contact tech@atcc.org. Thank you.
X

pRK2013 (ATCC® 37159)

Applications: mobilization helper plasmid  /  Depositors: EW Nester

Designations pRK2013
Permits and Restrictions

View Permits

Depositors EW Nester
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
Features:
marker(s): kanR
replicon: RK2
Applications
mobilization helper plasmid
Comments
This is a helper plasmid for mobilization of non-self-transmissible plasmids.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C
References

Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969

Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280

Shipped frozen