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pRK2013 (ATCC® 37159)

Applications: mobilization helper plasmid  /  Depositors: EW Nester

Designations pRK2013
Permits and Restrictions

View Permits

Depositors EW Nester
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Size (kb): 48.0
Vector: pRK2013 (plasmid)
Construction: pPRK212.2, pDF1
Marker(s): kanR
Construct size (kb): 48.0
marker(s): kanR
replicon: RK2
mobilization helper plasmid
This is a helper plasmid for mobilization of non-self-transmissible plasmids.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37°C

Knauf VC, Nester EW. Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium ti plasmid. Plasmid 8: 45-54, 1982. PubMed: 6292969

Figurski DH, Helinski DR. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76: 1648-1652, 1979. PubMed: 377280

Shipped frozen