Azospirillum brasilense Tarrand et al.

Potential plant growth promoting strain; Biofertilizer

1. Open vial according to enclosed instructions.
2. From a single tube of #838 broth (5 to 6 mL), withdraw approximately 0.6 to 1.0 mL with a Pasteur or 1.0 mL pipette and use to rehydrate the entire pellet.
3. Use 0.5 mL of this suspension to inoculate a #838 slant and 0.1 mL to inoculate #838 plates.
4. Incubate tubes and plates at 30°C, under aerobic conditions, for 48-72 hours.
5. After 48-72 hours of incubation, wash cells from the slant and transfer this broth to a new slant and plate.  Incubate another 48-72 hours under aerobic conditions.  This second transfer and incubation is necessary for complete removal of the cryoprotectant, which can inhibit growth.

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