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Azospirillum brasilense Tarrand et al. (ATCC® 29145)

Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Deposited As Spirillum lipoferum Beijerinck

Digitaria decumbens roots, plant, Brazil

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format freeze-dried
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Preceptrol® no
Type Strain yes
Nitrate reduction and nitrogenase activity
Symbiont with grasses in nitrogen fixation
L-arabinose metabolism
Exopolysaccharides and cyst formation
Carbon source metabolism
Medium ATCC® Medium 838: Spirillum nitrogen-fixing medium
Growth Conditions
Temperature: 30°C
Atmosphere: Aerobic
Name of Depositor J Dobereiner
Chain of Custody
ATCC <-- J Dobereiner <-- Sp. 7

Digitaria decumbens roots, plant, Brazil


Okon Y, et al. Growth of Spirillum lipoferum at constant partial pressures of oxygen, and the properties of its nitrogenase in cell-free extracts. J. Gen. Microbiol. 98: 87-93, 1977. PubMed: 13147

Validation list no. 2. Int. J. Syst. Bacteriol. 29: 79-80, 1979.

Neyra CA, Van Berkum P. Nitrate reduction nitrogenase activity in Spirillum lipoferum1. Can. J. Microbiol. 23: 306-310, 1977. PubMed: 856423

Tarrand JJ, et al. A taxonomic study of the Spirillum lipoferum group, with descriptions of a new genus, Azospirillum gen. nov. and two species, Azospirillum lipoferum (Beijerinck) comb. nov. and Azospirillum brasilense sp. nov. Can J Microbiol 24: 967-980, 1978. PubMed: 356945

Chakrabarti SK, et al. Cytochromes in Azospirillum brasilense. Curr. Microbiol. 11: 343-348, 1984.

Okon Y, et al. Carbon and ammonia metabolism of Spirillum lipoferum. J. Bacteriol. 128: 592-597, 1976. PubMed: 10278

Novick NJ, Tyler ME. L-arabinose metabolism in Azospirillum brasiliense. J. Bacteriol. 149: 364-367, 1982. PubMed: 6798025

Sadasivan L, Neyra CA. Flocculation in Azospirillum brasilense and Azospirillum lipoferum: exopolysaccharides and cyst formation. J Bacteriol 163: 716-723, 1985. PubMed: 3894333

Westby CA, et al. Metabolism of various carbon sources by Azospirillum brasilense. J. Bacteriol. 156: 1369-1372, 1983. PubMed: 6417113

Zhulin IB, et al. Oxygen taxis and proton motive force in Azospirillum brasilense. J. Bacteriol. 178: 5199-5204, 1996. PubMed: 8752338

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

Cross References

Nucleotide (GenBank) : X88853 A.brasilense ipdc gene (partial).

Nucleotide (GenBank) : Z37984 A.brasilense ntrB and ntrC genes.

Nucleotide (GenBank) : X99587 A.brasilense ipdC, gltX & cysS genes.

Nucleotide (GenBank) : X67216 A.brasilense pyrG gene for CTP synthetase.

Nucleotide (GenBank) : M64344 A.brasilense Fe protein and FeMo protein subunits and 353 bp orf, complete cds.

Nucleotide (GenBank) : M87319 Azospirillum brasilense dinitrogenase reductase ADP-ribosyl transferase (draT) gene and dinitrogenase reductase activating glycohydrolase (draG) gene, complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation