Mycobacterium simiae Karassova et al.

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet using approximately 0.5 mL of #1395 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1395 broth. Additional test tubes can be inoculated by transferring 0.5 mL from the primary test tubes to these secondary test tubes.
3. Use several drops from the primary test tube to inoculate a slant of #90 agar and/or a plate of #173 agar.
4. Incubate at 37°C for 9-28 days.

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