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Flavobacterium columnare (Bernardet and Grimont 1989) Bernardet et al. 1996

23463

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Flavobacterium columnare strain NCIMB 2248 is a bacterial type strain that was isolated from diseased chinook salmon from the Snake River in Washington.
Product category
Bacteria
Strain designation
NCIMB 2248 [I-S-2cl, NCMB 1038, CIP 103531, JCM 21141, LMG 13035, LMG 10406, NBRC 100251]
Type strain
Yes
Genome sequenced strain
Yes
Isolation source
Diseased chinook salmon (Oncorynchus tshawytscha) from Snake River
Geographical isolation
United States; Washington
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Preceptrol
No

Handling information

Medium
Temperature
20-24°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #1750 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #1750 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #1750 plate and/or #1750 agar slant.
  4. Incubate in a shaking incubator at 20-24° C for 3-6 days.
Handling notes
Establish growth in broth for 72 to 96 hours before transferring to agar.
The addition of 5% (v/v) heat inactivated fetal bovine serum to the broth media may aid in the recovery and growth of the organism following preservation. Two colony types may be observed on #1750 agar plates.
Growth is best achieved on a biphasic slant. Broth cultures do best if gently shaken. Culture exhibits light turbidity only. Maximum growth occurs at 5 to 6 days for RT cultures.
Culture grows best at 20°C. Room temperature is sufficient, providing it does not exceed 24°C to 26°C.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Cytophaga columnaris (Davis) Garnjobst
Depositors
NCIMB
Type of isolate
Animal
Cross references
GenBank AB078047 Flavobacterium columnare gene for 16S rRNA, strain: IFO 15943

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Bernardet JF, Grimont PA. Deoxyribonucleic acid related ness and phenotypic characterization of Flexibacter columnaris sp. nov., nom. rev., Flexibacter psychrophilus sp. nov., nom. rev., and Flexibacter maritimus Wakabayashi, Hikida, and Masumura. Int. J. Syst. Bacteriol. 39: 346-354, 1989.

Validation list no. 31. Int. J. Syst. Bacteriol. 39: 495-497, 1989.

Bernardet JF, et al. Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int. J. Syst. Bacteriol. 46: 128-148, 1996.

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