Pelomonas saccharophila (Doudoroff) Xie and Yokota

1. Open vial according to enclosed instructions or visit www.atcc.org for instructions.
2. Rehydrate the entire pellet with approximately 0.5 mL of #106 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #106 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
3. Use several drops of the primary broth tube to inoculate a #106 plate and/or #106 agar slant.
4. Incubate at 30°C for 72-96 hours.

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