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Quantitative Genomic DNA from Klebsiella aerogenes (ATCC® 13048DQ)

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Type Strain: yes  /  Biosafety Level: 1

Permits and Restrictions

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Application

ATCC® Genuine Nucleics can be used for assay development, verification, validation, monitoring of day-to-day test variation, and lot-to-lot performance of molecular-based assays. The quantitative format allows for the generation of a standard curve for quantitative PCR (qPCR) to determine bacterial load.

Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Specification range: ≥1 x 105 copies/µL
100 µL per vial
Storage Conditions -20°C or colder
Preceptrol® no
Type Strain yes
Comments Genomic DNA isolated from a preparation of Klebsiella aerogenes strain NCDC 819-56 (ATCC® 13048™). The source organism is also available through the ATCC catalog.

This strain is recommended by ATCC for use in the tests described in ASTM Standard Test Method D4783-89 where only the taxon is specified.
Name of Depositor ATCC
References

. . Dev. Ind. Microbiol. 20: 25-29, 1979.

ASTM International Standard Test Method for Resistance of Emulsion Paints in the Container to Attack by Microorganisms. West Conshohocken, PA:ASTM International;ASTM Standard Test Method D 2574-06.

Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.

quality control for the DuPont RiboPrinter™ Microbial Characterization System

RiboPrinter® QC Set. DuPont Nutrition & Health.

Enumeration of Escherichia coli and the Coliform Bacteria: LST-MUG Method for Detecting E.coli in chilled or Frozen Foods Exclusive of Bivalve Mollucan Sellfish. Washington, DC:US Food & Drug Administration;Bacteriological Analytical Manual Online Chapt. 4.II.3, 2002

Standard Test Method for Efficacy of Slimicides for the Paper Industry-Bacterial and Fungal Slime. West Conshohocken, PA:ASTM International;ASTM Standard Test Method E 1839-07.

Food Microbiology. Methods2.12: Examination for specific organisms--Escherichia coli in bivalve molluscs--Rapid method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 1766.2.12:2002.

Water microbiology. Method 4: Coliforms--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.4-1995.

Water microbiology. Method 5: Coliforms--Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.5-1995.

Water microbiology. Method 6: Thermotolerant coliforms and Escherichia coli--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.6-1995.

Water microbiology. Method 7: Thermotolerant coliforms and Escherichia coli -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.7-1995.

Water microbiology. Method 10: Pseudomonads--Estimation of most probable number (MPN). Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.10-1995.

Water microbiology. Method 11: Pseudomonads -- Membrane filtration method. Sydney, NSW, Australia:Standards Australia;Standards Australia AS 4276.11-1995.

Water microbiology. Method 18: Aeromonas by membrane filtration including selected speciation. Sydney, NSW, Australia:Standards Australia;Standards Australia AS/NZS 4276.18:2001.

Carpet Sanitizers. Washington, DC:Environmental Protection Agency;EPA EPA DIS/TSS-8, 1981

Sanitizer Test or Inanimate Surfaces. Washington, DC:Environmental Protection Agency;EPA EPA DIS/TSS-10.

Escherichia coli in Water by Membrane Filtration using membrane-Thermotolerant Escherichia coli Agar (mTEC). Washington, DC:Environmental Protection Agency;EPA EPA Method 1103.1, 2005

Escherichia coli in Water by Membrane Filtration Using Modified membrane-Thermotolerant Escherichia coli Agar. Washington, DC:Environmental Protection Agency;EPA EPA Method 1603, 2005

Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenation using Lauryl Tryptose Broth (LTB) and EC Medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1680, 2005

Fecal Coliforms in Sewage Sludge (Biosolids) by Multiple-Tube Fermenatation using A-1 medium. Washington, DC:Environmental Protection Agency;EPA EPA Method 1681, 2005

VITEK 2 BCL Comprehensive QC Set. bioMerieux.

VITEK 2 Comprehensive QC Set. bioMerieux.

RapID™ ONE QC Set. Remel™.

RapID™ Staph Plus QC Set. Remel™.

Cross References

Nucleotide (GenBank) : AX110938 Sequence 1671 from Patent WO0123604.

Nucleotide (GenBank) : AF052255 Enterobacter aerogenes DNA gyrase A (gyrA) gene, partial cds.

Nucleotide (GenBank) : AJ404624 Enterobacter aerogenes marA gene, marC gene (partial), marR gene and marB gene (partial).

Nucleotide (GenBank) : AB032468 Enterobacter aerogenes genes for ORF, L-2,4-diaminobutyrate decarboxylase, partial and complete cds.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
FAQs
  1. Accessing genome sequencing data


    Date Updated: 9/30/2019

  2. Criteria for reference quality genomes


    Date Updated: 9/30/2019