Genomic DNA from Clostridioides difficile strain 5325

This preparation of high molecular weight DNA is appropriate for use in the polymerase chain reaction (PCR)* process and other molecular biology applications. *the PCR process is covered by patents owned by Hoffmann-La Roche Inc. Use of the PCR process requires a license.

Presence of tcdA and tcdB genes confirmed by PCR.
Binary Toxin gene cdtB was amplified by PCR
Ribotype 078
Toxinotype V
PFGE type NAP7
REA type BK 16
Depositor-provided information: PFGE Type NAP7, Toxinotype V, and Binary Toxin positive.

1. Rehydrate contents of vial with molecular grade H₂O.
2. Place vial at 37°C for 1 hour or at 2°C to 8°C overnight.
3. For more complete rehydration and to fully recover DNA, incubate the sample overnight at 4°C while rocking; then incubate for 1 hour at 65°C.  Resuspending the dried DNA in ≥250 µL may give better results.

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.