Aneurinibacillus migulanus (Takagi et al.) Shida et al. emend. Heyndrickx et al.
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Takagi H, et al. Characterization of Bacillus brevis with descriptions of Bacillus migulanus sp. nov., Bacillus choshinensis sp. nov., Bacillus parabrevis sp. nov., and Bacillus galactophilus sp. nov.. Int. J. Syst. Bacteriol. 43: 221-231, 1993. PubMed: 8494737
Slapikoff S, et al. Sporulation in Bacillus brevis: studies on protease and protein turnover. J. Bacteriol. 106: 739-744, 1971. PubMed: 5557592
Hattman S, et al. Sequence specificity of DNA methylases from Bacillus amyloliquefaciens and Bacillus brevis. J. Mol. Biol. 124: 701-711, 1978. PubMed: 712853