Paenibacillus amylolyticus (Nakamura) Ash et al. emend. Shida et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
5. Incubate the tubes and plate at 30oC for 24 hours.
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Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.
Nakamura LK. Bacillus amylolyticus sp. nov., nom. rev., Bacillus lautus sp. nov., nom. rev. Bacillus pabuli sp. nov., nom. rev., and Bacillus validus sp. nov., nom. rev.. Int. J. Syst. Bacteriol. 34: 224-226, 1984.
Validation list no. 52. Int. J. Syst. Bacteriol. 45: 197-198, 1995.
The genus Bacillus, Agriculture Handbook 427. Washington, DC: ARS/USDA; 1973.
Shida O, et al. Emended description of Paenibacillus amylolyticus and description of Paenibacillus illinoisensis sp. nov. and Paenibacillus chibensis sp. nov.. Int. J. Syst. Bacteriol. 47: 299-306, 1997. PubMed: 9103613