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Asceptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well. Transfer 100 uL to a test tube containing 5 mL LB+50mg/mL of ampicillin. A loopful of culture can also be streaked on an LB + amp agar plate. Incubate cultures at 370 C. Isolate DNA using standard plasmid preparation procedures.
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Jelinek DF, et al. Cloning and regulation of cholesterol 7 alpha-hydroxylase, the rate-limiting enzyme in bile acid biosynthesis. J. Biol. Chem. 265: 8190-8197, 1990. PubMed: 2335522