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pCMV HSV S2P plasmid in Escherichia coli


This clone encodes human S2P with an HSV epitope tag at the amino terminus.

Depositor: Joseph L. Goldstein, Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX
Homo sapiens, human
Clone type
Molecular biology
Product format
Shipping information
Escherichia coli containing the plasmid
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
encodes an epitope tag for protein isolation or monitoring S2P


Restriction digests of the clone give the following sizes (kb): NheI/XbaI--5.4, 1.7; PstI--4.0, 3.1; ClaI--7.1; EcoRI--7.1.
The insert contains the following restriction sites (approximate kb from the 5' end): StyI--0.36; DraII--0.90; BglI--1.40; SacI--1.46.
The insert contains human S2P with HSV epitope tag at the amino terminus.
Mycoplasma contamination
Not detected

Vector information

Intact vector size
Vector name
Type of vector
ampR; G418
T7; SP6; CMV

Insert information

Insert size (kb)
Insert information
Gene: S2P
Source : human
Genbank accession: AF019612
Insert end 5’: NheI
Insert end 3’: XbaI

Handling information

Escherichia coli HB101 (ATCC 33694)
Handling procedure
  1. Open vial according to instructions.
  2. Asceptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well.  Transfer 100 µL to a test tube containing 5 mL LB+ ampicillin (50-100 µg/mL). A loopful of culture can also be streaked on an agar plate of the same. Incubate cultures at 37°C.
  3. Isolate DNA using standard plasmid preparation procedures.
Handling notes

Restriction digests of the clone gave the following sizes   (in kb):  NheI/XbaI – 5.4, 1.7 ; PstI – 4.0, 3.1 ; ClaI – 7.1 ; EcoRI – 7.1.                                                

–ATCC Staff


JL Goldstein
Special collection
NCRR Contract
Cross references
GenBank AF019612

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.


Frequently Asked Questions


Curated Citations

Rawson RB, et al. Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs. Mol. Cell 1: 47-57, 1997. PubMed: 9659902

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