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LL299 [SGC35242, U04343]

99597

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NotI--8.6; NotI/XhoI--7.2, 1.4; XhoI--8.6.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--0.02; HindIII--0.22; PstI--0.23; AccI--0.33; EcoRI--0.51, 0.66.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.800000190734863
Intact vector size
7.300
Vector name
pBJ
Type of vector
plasmid
Construction
pcDL-SRalpha296
Host range
mammalian cells
Vector end
XhoI; NotI
Vector information
other: SV40 late region polyA signal
Cloning sites
XhoI; XbaI; SfiI; NotI; EcoRI; EcoRV; HindIII; ClaI
Enhancer
HTLV-1 R-U5 segment of LTR
Markers
neoR; G418R; ampR
MCS
XhoI/.../ClaI, ->
Polylinker sites
XhoI; XbaI; SfiI; NotI; EcoRI; EcoRV; HindIII; ClaI
Promoters
SV40 early region
Replicon
SV40; pMB1

Insert information

Insert size (kb)
1.5
Type of DNA
cDNA
Insert information
DESCRIPTION OF INSERT COMPONENT:
ORF's seq. position: GDB probe: GDB:433597 ()
Insert 5' end: Modification: XhoI linker
Insert 3' end: Modification: NotI linker
Cross references: DNA Seq. Acc.: U04343
Nucleotides 1-1424 of the insert correspond to
nucleotides 1-1424 of U04343.
Genome
Homo sapiens
Chromosome
3
3, 544.330000 cR
Gene name
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
Gene product
CD86 antigen (CD28 antigen ligand 2, B7-2 antigen) [CD86]
Gene symbol
CD86; CD28LG2
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NotI--8.6;
NotI/XhoI--7.2, 1.4; XhoI--8.6.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): SacI--0.02; HindIII--0.22; PstI--0.23; AccI--0.33; EcoRI--0.51, 0.66.
- GenBank/EMBL/DDBJ

History

Depositors
LL Lanier
Cross references
GenBank U04343
GenBank 6380447

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Lin AY, et al. Expression of T cell antigen receptor heterodimers in a lipid-linked form. Science 249: 677-679, 1990. PubMed: 1696397

Lanier LL, et al. CD80(B7) and CD86(B70) provide similar costimulatory signals for T cell proliferation, cytokine production, and generation of CTL. J. Immunol. 154: 97-105, 1995. PubMed: 7527824

Azuma M, et al. B70 antigen is a second ligand for CTLA-4 and CD28. Nature 366: 76-79, 1993. PubMed: 7694153

Takebe Y, et al. SRalpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466-472, 1988. PubMed: 2827008

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