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LL288

99596

Detects:
Genome: Homo sapiens
Tissue: peripheral blood mononuclear cells
Gene symbol: CD94
Type of nucleic acid: genomic
Restriction enzyme: EcoRI
Invariant bands (kb): ~7.0, ~5.0
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item

Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BstXI--4.2; BstXI/NotI--3.4, 0.84; NotI--4.2.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.28; SacI--0.41.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.300000190734863
Intact vector size
3.500
Vector name
pJFE14
Type of vector
plasmid
Construction
pcDL-SRalpha296
Host range
mammalian cells
Vector end
BstXI; NotI
Cloning sites
BstXI; NotI; SacII; SfiI; EcoRI
Enhancer
HTLV-1 R-U5 segment of LTR
Markers
ampR
MCS
BstXI...EcoRI
Polylinker sites
BstXI; NotI; SacII; SfiI; EcoRI
Promoters
SV40 early region
Replicon
SV40; pMB1
Terminator
SV40 late region polyadenylation signal

Insert information

Insert size (kb)
0.80000000000000004
Type of DNA
cDNA
Insert source
polyclonal NK cell line
Insert tissue
polyclonal NK cell line
Insert information
DESCRIPTION OF INSERT COMPONENT:
ORF's seq. position: GDB probe: GDB:696221 ()
Insert 5' end: Modification: BstXI linker
Insert 3' end: Modification: NotI linker
Cross references: DNA Seq. Acc.: U30610
Nucleotides 1-834 of the insert correspond to
nucleotides 1-834 of U30610.
Genome
Homo sapiens
Chromosome
12
12 p13
Gene name
CD94 antigen (NK/T-cell C-type lectin receptor)
Gene product
CD94 antigen (NK/T-cell C-type lectin receptor) [CD94]
Gene symbol
CD94
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BstXI--4.2;
BstXI/NotI--3.4, 0.84; NotI--4.2.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): KpnI--0.28; SacI--0.41.
- GenBank/EMBL/DDBJ

History

Depositors
LL Lanier
Cross references
GenBank U30610
GenBank 6065180

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Chang C, et al. Molecular characterization of human CD94: a type II membrane glycoprotein related to the C-type lectin superfamily. Eur. J. Immunol. 25: 2433-2437, 1995. PubMed: 7589107

Lanier LL, et al. Human NKR-P1A. A disulfide-linked homodimer of the C-type lectin superfamily expressed by a subset of NK and T lymphocytes. J. Immunol. 153: 2417-2428, 1994. PubMed: 8077657

Takebe Y, et al. SRalpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol. 8: 466-472, 1988. PubMed: 2827008

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