ATCC ATCC Logo 0

pAQP1 Xenopus expression vector [UTR-01749, WI-9113, pCHIPev/BSII KS-]

99538

Detects:
Genome: Homo sapiens
Tissue: bone marrow
Gene symbol: AQP1
Type of nucleic acid: mRNA
Invariant bands (kb): 3.1
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Buy Now
Price: $567.00 EA
Discounts are available for our fellow nonprofit organizations.

Generally ships within 1-3 business days

Documentation

Haven't found what you need?

Customize solution

We tailor our materials for your specific application.

Get application-specific materials

Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
construct to generate mRNA that can be expressed in Xenopus oocytes aquaporin 1 (channel-forming integral protein, 28 kD)

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.3, 1.1, 0.22; HindIII/PstI--2.8, 1.2; PvuII--2.4, 0.78, 0.6, 0.22.
Insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.08, 0.27; PvuII--0.34, 0.53.
The AQP1 coding sequence is flanked by 56 bp 5' untranslated sequence and 210 bp 3' untranslated sequence from the Xenopus beta-globin gene.
Sense mRNA can be synthesized in vitro from the SmaI digested contruct using T3 RNA polymerase. Resulting mRNA can be injected into and expressed in Xenopus oocytes.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.057000160217285
Intact vector size
2.961
Vector name
pBluescript II KS-
Type of vector
phagemid
Host range
Escherichia coli
Vector end
HindIII; PstI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ', <-, 816-938
Markers
ampR
MCS
SacI...KpnI, ->, 657-759
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Primer site
M13-20, ->; reverse, <-
Promoters
T7; T3
Replicon
f1, →, 3-459; pMB1, 1032-1972

Insert information

Insert size (kb)
1.1140000000000001
Type of DNA
cDNA
Insert source
bone marrow
Insert tissue
Bone marrow
Insert information
DESCRIPTION OF INSERT COMPONENT:
Insert 5' end: HindIII
Insert 3' end: PstI
Cross references: DNA Seq. Acc.: M77829
Nucleotides 57-904 of the insert correspond to
nucleotides ~1-860 of M77829.
Genome
Homo sapiens
Chromosome
7
7 p14; 7, 84.8799999 cR
Target gene
aquaporin 1 (channel-forming integral protein, 28 kD)
Gene name
aquaporin 1 (channel-forming integral protein, 28 kD)
Gene product
aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1]
Gene symbol
AQP1; CHIP28
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli DH5alpha
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BglI--1.5, 1.3,
1.1, 0.22; HindIII/PstI--2.8, 1.2; PvuII--2.4, 0.78, 0.6, 0.22.
- ATCC staff

Insert contains the following restriction sites (approximate kb from the 5'
end): BglI--0.08, 0.27; PvuII--0.34, 0.53.
- GenBank/EMBL/DDBJ

The AQP1 coding sequence is flanked by 56 bp 5' untranslated sequence and 210 bp
3' untranslated sequence from the Xenopus beta-globin gene.
- personal communication

Sense mRNA can be synthesized in vitro from the SmaI digested contruct using T3
RNA polymerase. Resulting mRNA can be injected into and expressed in Xenopus
oocytes.
- Science 256: 385-387, 1992

History

Depositors
P Agre, GM Preston
Special collection
NCRR Contract
Cross references
GenBank M77829
GenBank 3785830

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319

Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524

Peter Agre, personal communication

Frequently Asked Questions

Need assistance with this product? Contact our Technical Support team.

Telephone

Telephone

US and Puerto Rico

800-638-6597

Outside the US

+1-703-365-2700
hours

Hours of Operation

Monday-Friday
9:30am - 5:30pm
US Eastern Time