ATCC ATCC Logo 0

pG28B5.0

99536

Detects:
Genome: Homo sapiens
Tissue: placenta, liver, lung, skeletal muscle, pancreas, small
intestine, colon, peripheral blood leukocytes, heart,
spleen and prostate
Gene symbol: CEBPA
Type of nucleic acid: mRNA
Invariant bands (kb): 2.7
Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence CCAAT/enhancer binding protein (C/EBP), alpha

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.2; HindIII--6.2, 1.5, 0.46; EcoRI--5.6, 2.5.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--3.0; EcoRI--2.5; EcoRV--3.2; HindIII--3.1, 4.6; NcoI--1.3, 2.3; NotI--1.65, 1.7; PstI--0.7, 1.3, 1.85, 1.9, 4.4; SacI--2.3, 3.9; XbaI--3.5; XhoI--0.2, 2.35.
The probe used for Northern blotting was the 0.7 kb EcoRI/HindIII fragment from the 3' untranslated region of the insert.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.199999809265137
Intact vector size
3.199
Vector name
pGEM-3Zf(+)
Type of vector
phagemid
Host range
Escherichia coli
Vector end
BamHI
Cloning sites
EcoRI; SacI; KpnI; AvaI; SmaI; BamHI; XbaI; SalI; AccI; HincII; PstI; SphI; HindIII
Insert detection
lacZ'
Markers
ampR
Polylinker sites
T7-EcoRI; SacI; KpnI; AvaI; SmaI; BamHI; XbaI; SalI; AccI; HincII; PstI; SphI; HindIII-SP6
Promoters
lac; SP6; T7
Replicon
pMB1; f1

Insert information

Insert size (kb)
5
Type of DNA
genomic
Insert source
umbilical vein endothelial cells
Insert tissue
umbilical vein endothelial cells
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: U34070
Nucleotides ~700-4000 of the insert correspond to
nucleotides 1-3318 of U34070.
Genome
Homo sapiens
Chromosome
19
19 q13.1
Target gene
CCAAT/enhancer binding protein (C/EBP), alpha
Gene name
CCAAT/enhancer binding protein (C/EBP), alpha
Gene product
CCAAT/enhancer binding protein (C/EBP), alpha [CEBPA]
Gene symbol
CEBPA
Contains complete coding sequence
Yes
Insert end
BamHI

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--5.0, 3.2;
HindIII--6.2, 1.5, 0.46; EcoRI--5.6, 2.5.
- ATCC staff

The probe used for Northern blotting was the 0.7 kb EcoRI/HindIII fragment from
the 3' untranslated region of the insert.
- Biochem. Biophys. Res. Commun. 215: 106-113, 1995

Insert contains the following restriction sites (approximate kb from the 5'
end): BglII--3.0; EcoRI--2.5; EcoRV--3.2; HindIII--3.1, 4.6; NcoI--1.3, 2.3;
NotI--1.65, 1.7; PstI--0.7, 1.3, 1.85, 1.9, 4.4; SacI--2.3, 3.9; XbaI--3.5;
XhoI--0.2, 2.35.
- Biochem. Biophys. Res. Commun. 215: 106-113, 1995

History

Depositors
KG Xanthopoulos
Special collection
NCRR Contract
Cross references
GenBank U34070
GenBank 686326

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Antonson P, Xanthopoulos KG. Molecular cloning, sequence, and expression patterns of the human gene encoding CCAAT/enhancer binding protein alpha (C/EBP alpha). Biochem. Biophys. Res. Commun. 215: 106-113, 1995. PubMed: 7575576

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