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pCWA274

99478

Detects:
Genome: Homo sapiens
Gene symbol: DNA-PK(CS)
Type of nucleic acid: genomic
Restriction enzyme: PvuII
Number of alleles: 2
Type of polymorphism: site
Alleles:
Allele Freq. Size (kb) Strains
- 0.32 1.361
+ 0.68 0.870 0.491
Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0; BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4.
The insert contains the following restriction sites (approximate kb from the 5' end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01.
PvuII polymorphism was detected in the PCR product generated with upstream primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt 7570-7589).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
10.35000038146973
Intact vector size
2.964
Vector name
pBluescript KS+
Type of vector
phagemid
Construction
pUC19
Host range
Escherichia coli
Vector end
EcoRI
Cloning sites
SacII; XmaII; NotI; XbaI; SpeI; BamHI; SmaI; PstI; EcoRI; EcoRV; HindIII; ClaI; SalI HincII AccI; XhoI; DraII; ApaI; KpnI
Enhancer
none
Insert detection
lacZ'
Markers
ampR
Polylinker sites
SEE COMMENTS
Promoters
lac; T3; T7
Replicon
pMB1; f1
Terminator
none

Insert information

Insert size (kb)
7.3920000000000003
Type of DNA
genomic
Insert information
DESCRIPTION OF INSERT COMPONENT:
subunit)
Cross references: DNA Seq. Acc.: L27425
Nucleotides 1-7393 of the insert correspond to
nucleotides 247-7693 of L27425.
Genome
Homo sapiens
Chromosome
8
8 q11
Gene name
DNA-activated serine/threonine protein kinase (catalytic
Gene product
DNA-activated serine/threonine protein kinase (catalytic subunit) [DNA-PK(CS)]
Gene symbol
DNA-PK(CS)
Alleles
-, +
Contains complete coding sequence
No
Insert end
EcoRI

Handling information

Host
Escherichia coli DH5alpha
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--7.4, 3.0;
BamHI--10.4; BglII--9.3, 1.05; HindIII--6.0, 4.4; SmaI--8.0, 2.4.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): SacI--3.06, 5.68; BglII--5.15, 6.00; SmaI--5.26; HindIII--6.01.
- GenBank/EMBL/DDBJ

PvuII polymorphism was detected in the PCR product generated with upstream
primer (nt 6229-6252 of L27425) and downstream primer (compliment of nt
7570-7589).
- Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995

History

Depositors
CW Anderson
Cross references
GenBank L27425

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Sipley JD, et al. Gene for the catalytic subunit of the human DNA-activated protein kinase maps to the site of the XRCC7 gene on chromosome 8. Proc. Natl. Acad. Sci. USA 92: 7515-7519, 1995. PubMed: 7638222

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