ATCC ATCC Logo 0

yRM2006

99425

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Contains sequences encoding V(H)2-1, 4-4, and 5-1.
Most of the insert sequence is also available in cosmid clones: yIgH6c1-5, 9-10, 14, 18, and 19 (ATCC 99450, 99452, 99454, 99456, 99472, 99458, 99460, 99462, 99464, 99466, respectively).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
210.0
Intact vector size
9.000
Vector name
pTYAC1
Type of vector
YAC
Host range
Saccharomyces cerevisiae
Escherichia coli
Vector end
EcoRI
Vector information
other: telomere, Tetrahymena, <-
Centromere
CEN4
Cloning sites
EcoRI
Markers
ampR; URA3
Replicon
ARS1; pMB1
Restriction sites
EcoRI

Insert information

Insert size (kb)
200
Type of DNA
genomic
Insert source
leukocyte
Insert tissue
leukocyte
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
Cross references: DNA Seq. Acc.: Z27502
DNA Seq. Acc.: Z27503
DNA Seq. Acc.: Z27504
DNA Seq. Acc.: Z27505
DNA Seq. Acc.: Z27506
DNA Seq. Acc.: Z27507
DNA Seq. Acc.: Z27508
DNA Seq. Acc.: Z27509
DNA Seq. Acc.: Z27510
DNA Seq. Acc.: Z29986
Genome
Homo sapiens
Chromosome
14
14 q32.33; 14 q32.3; 14 q32.3-qter
Gene name
immunoglobulin heavy polypeptide, variable region (cluster); DNA Segment, single copy; DNA Segment, repetitive (telomere)
Gene product
immunoglobulin heavy-chain variable region [[email protected]]
Gene symbol
[email protected]; D14S308
Contains complete coding sequence
No
Insert end
EcoRI

Handling information

Host
Saccharomyces cerevisiae AB1380 (ATCC 20843)
Temperature
30°C
Handling notes
Contains sequences encoding V(H)2-1, 4-4, and 5-1.
- Nat. Genet. 7: 162-168, 1994

Most of the insert sequence is also available in cosmid clones: yIgH6c1-5, 9-10,
14, 18, and 19 (ATCC 99450, 99452, 99454, 99456, 99472, 99458, 99460, 99462,
99464, 99466, respectively).
- Nat. Genet. 7: 162-168, 1994

History

Depositors
HC Riethman
Cross references
GenBank Z27502
GenBank Z27503
GenBank Z27504
GenBank Z27505
GenBank Z27506
GenBank Z27507
GenBank Z27508
GenBank Z27509
GenBank Z27510
GenBank Z29986
GenBank 229851
GenBank 373988

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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