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pPW71B [PW71B]

99413

Detects:
Genome: Homo sapiens
Gene symbol: D15S63
Type of nucleic acid: dsDNA
Restriction enzyme: HindIII+HpaII
Number of alleles: 2
Type of polymorphism: parental methylation
Alleles:
Allele Freq. Size (kb) Strains
4.7 paternal chromosomes
6.6 maternal chromosomes
Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Dried
Shipping information
Rehydrate with TE
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence useful for DNA diagnostics DNA Segment, single copy probes

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2.
Insert detects parent-of-origin specific DNA methylation patterns in the Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An imprinted HpaII and CfoI site in this region results in the maternal and paternal pattern.
Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of the maternal bands is indicative of Angelman syndrome. Because of the small probe size, low-stringent post-hybridization washes should be applied.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.049999952316284
Intact vector size
2.686
Vector name
pUC19
Type of vector
plasmid
Construction
pUC71K
Host range
Escherichia coli
Vector end
SmaI
Vector information
Cross references: DNA Seq. Acc.: X02514
Cloning sites
EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; HincII; AccI; SalI; PstI; SphI; HindIII
Insert detection
lacZ', <-
Markers
ampR
MCS
EcoRI...HindIII, ->
Polylinker sites
EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; HincII; AccI; SalI; PstI; SphI; HindIII
Replicon
pMB1, <-

Insert information

Insert size (kb)
0.36499999999999999
Type of DNA
genomic
Genome
Homo sapiens
Chromosome
15
15 q11-q12
Target gene
DNA Segment, single copy probes
Gene name
DNA Segment, single copy probes
Gene product
DNA Segment, single copy probes [D15S63]
Gene symbol
D15S63
Contains complete coding sequence
No
Insert end
HaeIII

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb):
EcoRI/HindIII--2.8, 0.44; PstI--3.2, PvuII--2.5, 0.76; SacI--3.2; XbaI--3.2.
- ATCC staff

Insert detects parent-of-origin specific DNA methylation patterns in the
Prader-Willi syndrome/Angelman syndrome chromosomal region (15 q11-q13). An
imprinted HpaII and CfoI site in this region results in the maternal and
paternal pattern.
- Hum. Mol. Genet. 2: 1995-1999, 1993

Patients with Prader-Willi syndrome typically lack the paternal bands. Lack of
the maternal bands is indicative of Angelman syndrome. Because of the small
probe size, low-stringent post-hybridization washes should be applied.
- Hum. Mol. Genet. 2: 1995-1999, 1993

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Dittrich B, et al. Characterization of a methylation imprint in the Prader-Willi syndrome chromosome region. Hum. Mol. Genet. 2: 1995-1999, 1993. PubMed: 8111366

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