Providencia rettgeri (Hadley et al.) Brenner et al.
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 37°C for 24 hours.
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Huang HT, English AR. Production of 6-aminopenicillanic acid. US Patent 3,239,427 dated Mar 8 1966
Huang HT. 6-Aminopenicillanic acid amide production. US Patent 3,088,880 dated May 7 1963
. . J. Bacteriol. 49: 419-437, 1945.
. . J. Bacteriol. 49: 437-444, 1945.