Giesbergeria sinuosa (Williams and Rittenberg) Grabovich et al.
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Incubate at 26oC for 72 hours.
5. After growth has been achieved, use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
Colonies on Nutrient Agar are entire, glistening, smooth, drop-like, pinpoint, and translucent.
Additional information on this culture is available on the ATCC web site at www.atcc.org.
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Williams MA, Rittenberg SC. A taxonomic study of the genus Spirillum Ehrenberg. Int. Bull. Bacteriol. Nomencl. Taxon. 7: 49-110, 1957.
Hylemon PB, et al. The genus Spirillum: a taxonomic study. Int. J. Syst. Bacteriol. 23: 340-380, 1973.
Skerman VB, et al. Approved lists of bacterial names. Int J Syst Bacteriol 30: 225-420, 1980.