Hafnia alvei Moller
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2. From a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Use several drops of this suspension to inoculate an additional broth tube, an agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
Colonies on #3 agar are entire, glistening, smooth, and translucent.
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Yokozeki K, Kubota K. Method of producing L-carnitine. US Patent 4,650,759 dated Mar 17 1987
. . J. Biol. Chem. 156: 401-409, 1944.