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p58-CL39 [SGC35391, U24077]

95700

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4, 1.6; EcoRV--5.7; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.8, 1.4, 0.6, 0.1; XhoI--5.7.
The insert contains the following restriction sites (approximate kb from the 5' end): BclI--0.35; PstI--0.04, 0.43, 0.51, 0.84; PvuII--1.12; SacI--0.45; StuI--1.05; XhoI--0.62.
Encodes a variant not recognized by either monoclonal antibody GL183 or EB6.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.670000076293945
Intact vector size
4.109
Vector name
pSPORT1
Type of vector
plasmid
Host range
Escherichia coli
Vector end
NotI; SalI
Cloning sites
AatII; SphI; mLuI; SunI; SnaBI; HindIII; BamHI; XbaI; XmaIII; NotI; SpeI; SstI; SalI; AccI; SmaI; EcoRI; Kpn2I; RsrII; PinAI; KpnI; Sse8387I; PstI
Insert detection
lacZ', <-, 1-341
Markers
ampR
MCS
AatII...PstI, ->, 191-285
Polylinker sites
AatII; SphI; mLuI; SunI; SnaBI; HindIII; BamHI; XbaI; XmaIII; NotI; SpeI; SstI; SalI; AccI; SmaI; EcoRI; Kpn2I; RsrII; PinAI; KpnI; Sse8387I; PstI
Promoters
SP6; T7
Replicon
pMB1, 1903-1903
Repressor gene
lacI, <-, 464-1546

Insert information

Insert size (kb)
1.5660000000000001
Type of DNA
cDNA
Insert source
Resting CD3- cells
Insert tissue
Resting CD3- cells
Insert information
DESCRIPTION OF INSERT COMPONENT:
ORF's seq. position: GDB probe: GDB:698358 ()
Insert 5' end: Modification: SalI linker
Insert 3' end: Modification: NotI linker
Cross references: DNA Seq. Acc.: U24077
Nucleotides 1-1532 of the insert correspond to
nucleotides 1-1532 of U24077.
Genome
Homo sapiens
Chromosome
19
19 q13.4; 19, 374.860000 cR
Gene name
killer cell inhibitory receptor
Gene product
killer cell inhibitory receptor [KIR]
Gene symbol
KIR; NKB1
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): NotI/SalI--4.4,
1.6; EcoRV--5.7; PstI--5.0, 0.4, 0.35, 0.1; PvuII--3.8, 1.4, 0.6, 0.1;
XhoI--5.7.
- ATCC staff

Encodes a variant not recognized by either monoclonal antibody GL183 or EB6.
- Immunity 2: 439-449, 1995

The insert contains the following restriction sites (approximate kb from the 5'
end): BclI--0.35; PstI--0.04, 0.43, 0.51, 0.84; PvuII--1.12; SacI--0.45;
StuI--1.05; XhoI--0.62.
- Immunity 2: 439-449, 1995

.nichd

History

Depositors
N Wagtmann
Cross references
GenBank U24077

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Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Wagtmann N, et al. Molecular clones of the p58NK cell receptor reveal immunoglobulin-related molecules with diversity in both the extra- and intracellular domains. Immunity 2: 439-449, 1995. PubMed: 7749980

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