ATCC ATCC Logo 0

pHKHK1-2

95693

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Dried
Shipping information
Rehydrate with TE
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
The first polyadenylation signal was used.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): XbaI--4.2, 1.2; HindIII--5.4; BamHI--3.8, 1.6; PstI--4.5, 0.5, 0.4; SacI--4.8, 0.5.
Derived from the form C variant transcript (alternately spliced third coding exon). The first polyadenylation signal was used.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.429999828338623
Intact vector size
4.356
Vector name
pCDM8
Type of vector
phagemid
Construction
CMV, SV40
Host range
vertebrate cells
Vector end
BstXI
Cloning sites
HindIII; XbaI; XhoI; BstXI; PstI; NotI
Markers
ampR; supF
MCS
HindIII...XbaI, ->, 2198-2655
Polylinker sites
HindIII; XbaI; XhoI; BstXI; --stuffer--BstXI; XhoI; PstI; NotI; XbaI
Promoters
CMV; T7
Replicon
pMB1, 1-587; M13, ←, 588-1182; polyoma, 3353-4188; SV40, 4189-4352
Terminator
SV40 splice site and polyadenylation, ->, 2656-3352

Insert information

Insert size (kb)
0.93100000000000005
Type of DNA
cDNA
Insert source
Hep G2 cell line
Insert tissue
Hep G2 cell line
Insert information
DESCRIPTION OF INSERT COMPONENT:
Disease: hepatocellular carcinoma
Cross references: DNA Seq. Acc.: X78677
Nucleotides 1-931 of the insert correspond to
Disease: hepatocellular carcinoma
nucleotides 1-931 of X78677.
Genome
Homo sapiens
Gene name
ketohexokinase (fructokinase)
Gene product
ketohexokinase (fructokinase) [KHK]
Gene symbol
KHK
Contains complete coding sequence
No

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): XbaI--4.2, 1.2;
HindIII--5.4; BamHI--3.8, 1.6; PstI--4.5, 0.5, 0.4; SacI--4.8, 0.5.
- ATCC staff

Derived from the form C variant transcript (alternately spliced third coding
exon). The first polyadenylation signal was used.
- Hum. Mol. Genet. 3: 1627-1631, 1994

The insert contains the following restriction sites (approximate kb from the 5'
end): BamHI--0.2; PstI--0.24, 0.67; SacI--0.33.
- Hum. Mol. Genet. 3: 1627-1631, 1994

History

Cross references
GenBank X78677

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Bonthron DT, et al. Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase). Hum. Mol. Genet. 3: 1627-1631, 1994. PubMed: 7833921

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