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pAQP1 [UTR-01749, WI-9113, pCHIP]

95675

Detects:
Genome: Homo sapiens
Tissue: bone marrow
Gene symbol: AQP1
Type of nucleic acid: mRNA
Invariant bands (kb): 3.1
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Dried
Shipping information
Rehydrate with TE
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8; HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.80; HindIII--1.28.
The insert begins at nt +15 relative to the initiation ATG, and contains about 1.3 kb at the 3' end that has not been sequenced.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.760000228881836
Intact vector size
2.961
Vector name
pBluescript II KS-
Type of vector
phagemid
Host range
Escherichia coli
Vector end
EcoRI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ', <-, 816-938
Markers
ampR
MCS
SacI...KpnI, ->, 657-759
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Primer site
M13-20, ->; reverse, <-
Promoters
T3; T7
Replicon
f1, →, 3-459; pMB1, 1032-1972

Insert information

Insert size (kb)
2.7999999999999998
Type of DNA
cDNA
Insert source
bone marrow
Insert tissue
Bone marrow
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: M77829
Nucleotides 1-1283 of the insert correspond to
nucleotides 53-1335 of M77829.
Genome
Homo sapiens
Chromosome
7
7 p14; 7, 84.8799999 cR
Gene name
aquaporin 1 (channel-forming integral protein, 28 kD)
Gene product
aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1]
Gene symbol
AQP1; CHIP28
Contains complete coding sequence
No
Insert end
Modification: EcoRI linker

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8;
HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): SmaI--0.80; HindIII--1.28.
- GenBank/EMBL/DDBJ

The insert begins at nt +15 relative to the initiation ATG, and contains about
1.3 kb at the 3' end that has not been sequenced.
- Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991

History

Cross references
GenBank M77829

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319

Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524

Preston GM, et al. The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel. J. Biol. Chem. 268: 17-20, 1993. PubMed: 7677994

Bondy C, et al. Developmental gene expression and tissue distribution of the CHIP28 water-channel protein. Proc. Natl. Acad. Sci. USA 90: 4500-4504, 1993. PubMed: 8506291

Moon C, et al. The human aquaporin-CHIP gene. J. Biol. Chem. 268: 15772-15778, 1993. PubMed: 8340403

View All Curated Citations for this Product

Frequently Asked Questions

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