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pAQP1 [UTR-01749, WI-9113, pCHIP]

95674

Detects:
Genome: Homo sapiens
Tissue: bone marrow
Gene symbol: AQP1
Type of nucleic acid: mRNA
Invariant bands (kb): 3.1
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Mission Collection Item
This is a Mission Collection Item

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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8; HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8.
The insert contains the following restriction sites (approximate kb from the 5' end): SmaI--0.80; HindIII--1.28.
The insert begins at nt +15 relative to the initiation ATG, and contains about 1.3 kb at the 3' end that has not been sequenced.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.760000228881836
Intact vector size
2.961
Vector name
pBluescript II KS-
Type of vector
phagemid
Host range
Escherichia coli
Vector end
EcoRI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ', <-, 816-938
Markers
ampR
MCS
SacI...KpnI, ->, 657-759
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Primer site
M13-20, ->; reverse, <-
Promoters
T3; T7
Replicon
f1, →, 3-459; pMB1, 1032-1972

Insert information

Insert size (kb)
2.7999999999999998
Type of DNA
cDNA
Insert source
bone marrow
Insert tissue
Bone marrow
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: M77829
Nucleotides 1-1283 of the insert correspond to
nucleotides 53-1335 of M77829.
Genome
Homo sapiens
Chromosome
7
7 p14; 7, 84.8799999 cR
Gene name
aquaporin 1 (channel-forming integral protein, 28 kD)
Gene product
aquaporin 1 (channel-forming integral protein, 28 kD) [AQP1]
Gene symbol
AQP1; CHIP28
Contains complete coding sequence
No
Insert end
Modification: EcoRI linker

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.8;
HindIII--4.4, 1.5; SmaI--5.0, 0.8; KpnI--5.8; BglII--5.8.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): SmaI--0.80; HindIII--1.28.
- GenBank/EMBL/DDBJ

The insert begins at nt +15 relative to the initiation ATG, and contains about
1.3 kb at the 3' end that has not been sequenced.
- Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991

History

Depositors
P Agre
Cross references
GenBank M77829
GenBank 183925

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Preston GM, Agre P. Isolation of the cDNA for erythrocyte integral membrane protein of 28 kilodaltons: member of an ancient channel family. Proc. Natl. Acad. Sci. USA 88: 11110-11114, 1991. PubMed: 1722319

Preston GM, et al. Appearance of water channels in Xenopus oocytes expressing red cell CHIP28 protein. Science 256: 385-387, 1992. PubMed: 1373524

Preston GM, et al. The mercury-sensitive residue at cysteine 189 in the CHIP28 water channel. J. Biol. Chem. 268: 17-20, 1993. PubMed: 7677994

Bondy C, et al. Developmental gene expression and tissue distribution of the CHIP28 water-channel protein. Proc. Natl. Acad. Sci. USA 90: 4500-4504, 1993. PubMed: 8506291

Moon C, et al. The human aquaporin-CHIP gene. J. Biol. Chem. 268: 15772-15778, 1993. PubMed: 8340403

View All Curated Citations for this Product

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