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pGEM-8

95660

Detects:
Genome: Homo sapiens
Gene symbol: IGF2R
Type of nucleic acid: genomic
Restriction enzyme: SacI
Invariant bands (kb): 2.2, 3.1, 4.0, 4.9, 5.3
Number of alleles: 2
Maximum heterozygosity (%): 52
Alleles:
Allele Freq. Size (kb) Strains
A1 0.35 14.4
A1 0.65 18.6
Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Frozen
Mission Collection Item
This is a Mission Collection Item

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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0, 1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7; SalI/XhoI--9.0, 2.7.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34, 6.91.
At the 3' end of the insert, the construct includes sequence from the vector pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation signal (to the SalI site).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
12.0
Intact vector size
2.870
Vector name
pGEM-2
Type of vector
plasmid
Host range
Escherichia coli
Vector end
SalI; XbaI
Vector information
Cross references: DNA Seq. Acc.: X65301
Cloning sites
HindIII; PstI; SalI; AccI; HincII; XbaI; BamHI; AvaI; SmaI; SacI; EcoRI
Markers
ampR
MCS
EcoRI...HindIII, ->, 10-60
Polylinker sites
HindIII; PstI; SalI; AccI; HincII; XbaI; BamHI; AvaI; SmaI; SacI; EcoRI
Promoters
T7; SP6
Replicon
pMB1

Insert information

Insert size (kb)
9.0999999999999996
Type of DNA
cDNA
Insert source
placenta
Insert tissue
placenta
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
Insert 5' end: SalI
Insert 3' end: XbaI
Cross references: DNA Seq. Acc.: J03528
Genome
Homo sapiens
Chromosome
6
6 q25.3
Gene name
insulin-like growth factor 2 receptor
Gene product
insulin-like growth factor 2 receptor( insulin-like growth factor II receptor, insulin like growth factor II (IGF II) receptor, mannose-6-phosphate receptor, cation-independent) [IGF2R]
Gene symbol
IGF2R
Alleles
A1, A1
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli DH5alpha
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0,
1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7;
SalI/XhoI--9.0, 2.7.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34,
6.91.
- GenBank/EMBL/DDBJ

At the 3' end of the insert, the construct includes sequence from the vector
pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation
signal (to the SalI site).
- personal communication

History

Depositors
WS Sly
Cross references
GenBank J03528

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Intended use
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Oshima A, et al. The human cation-independent mannose 6-phosphate receptor. Cloning and sequence of the full-length cDNA and expression of functional receptor in COS cells. J. Biol. Chem. 263: 2553-2562, 1988. PubMed: 2963003

William S Sly, personal communication

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