pGEM-8

95660 ^™

Detects:
Genome: Homo sapiens
Gene symbol: IGF2R
Type of nucleic acid: genomic
Restriction enzyme: SacI
Invariant bands (kb): 2.2, 3.1, 4.0, 4.9, 5.3
Number of alleles: 2
Maximum heterozygosity (%): 52
Alleles:
Allele Freq. Size (kb) Strains
A1 0.35 14.4
A1 0.65 18.6

Organism: Homo sapiens, human
Clone type: Clone
Applications: Molecular biology
Product format: Frozen

See Additional Product Information

Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0, 1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7; SalI/XhoI--9.0, 2.7.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34, 6.91.
At the 3' end of the insert, the construct includes sequence from the vector pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation signal (to the SalI site).
Mycoplasma contamination: Not detected

Vector information

Construct size (kb): 12.0
Intact vector size: 2.870
Vector name: pGEM-2
Type of vector: plasmid
Host range: Escherichia coli
Vector end: SalI; XbaI
Vector information: Cross references: DNA Seq. Acc.: X65301
Cloning sites: HindIII; PstI; SalI; AccI; HincII; XbaI; BamHI; AvaI; SmaI; SacI; EcoRI
Markers: ampR
MCS: EcoRI...HindIII, ->, 10-60
Polylinker sites: HindIII; PstI; SalI; AccI; HincII; XbaI; BamHI; AvaI; SmaI; SacI; EcoRI
Promoters: T7; SP6
Replicon: pMB1

Insert information

Insert size (kb): 9.0999999999999996
Type of DNA: cDNA
Insert source: placenta
Insert tissue: placenta
Insert information: DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
Insert 5' end: SalI
Insert 3' end: XbaI
Cross references: DNA Seq. Acc.: J03528
Genome: Homo sapiens
Chromosome: 6

6 q25.3
Gene name: insulin-like growth factor 2 receptor
Gene product: insulin-like growth factor 2 receptor( insulin-like growth factor II receptor, insulin like growth factor II (IGF II) receptor, mannose-6-phosphate receptor, cation-independent) [IGF2R]
Gene symbol: IGF2R
Alleles: A1, A1
Contains complete coding sequence: Yes

Handling information

Host: Escherichia coli DH5alpha
Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling notes: Restriction digests of the clone give the following sizes (kb): BamHI--6.6, 4.0,
1.8; EcoRI--8.0, 3.7; HindIII--5.0, 4.0, 1.8, 1.5; SalI--9.0, 2.7;
SalI/XhoI--9.0, 2.7.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): EcoRI--0.48, 8.40; HindIII--0.72, 2.24, 3.94; BamHI--2.81, 2.96, 3.34,
6.91.
- GenBank/EMBL/DDBJ

At the 3' end of the insert, the construct includes sequence from the vector
pSLV: XbaI, SmaI, SacI, and BamHI sites, and the SV40 poly(A) polyadenylation
signal (to the SalI site).
- personal communication

History

Depositors: WS Sly
Cross references: GenBank J03528

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

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Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Oshima A, et al. The human cation-independent mannose 6-phosphate receptor. Cloning and sequence of the full-length cDNA and expression of functional receptor in COS cells. J. Biol. Chem. 263: 2553-2562, 1988. PubMed: 2963003

William S Sly, personal communication

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