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GRK4A

95519

Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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Documentation

Biosafety Icon Biosafety Level 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence G protein-coupled receptor kinase 2 (Drosophila)-like G protein-coupled receptor kinase 4

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0, 0.2; EcoRI--3.2; BamHI--3.2; HindIII--3.2; XhoI--3.2.
Insert encodes the N-terminal portion of the GRK4A isoform. It contains an in-frame 96 bp insertion following codon 17 of the isoform GRK4B sequence.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.200000047683716
Intact vector size
2.961
Vector name
pBluescript II SK+
Type of vector
phagemid
Host range
Escherichia coli
Vector end
XhoI; EcoRI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ'
Markers
ampR
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Promoters
T7; T3; lac
Replicon
pMB1; f1

Insert information

Insert size (kb)
0.24299999999999999
Type of DNA
cDNA
Insert source
brain
Insert tissue
brain
Insert information
DESCRIPTION OF INSERT COMPONENT:
Insert 5' end: XhoI
Insert 3' end: EcoRI
Cross references: DNA Seq. Acc.: X75897
Nucleotides 1-243 of the insert correspond to
nucleotides 1-243 of X75897.
Genome
Homo sapiens
Chromosome
4
4 p16.3
Target gene
G protein-coupled receptor kinase 2 (Drosophila)-like
Gene name
G protein-coupled receptor kinase 2 (Drosophila)-like
Gene product
G protein-coupled receptor kinase 2 (Drosophila)-like(G protein-coupled receptor kinase 4) [GRK4]
Gene symbol
GPRK2L; GPRK4; GRK4
Contains complete coding sequence
No

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--3.0,
0.2; EcoRI--3.2; BamHI--3.2; HindIII--3.2; XhoI--3.2.
- ATCC staff

Insert encodes the N-terminal portion of the GRK4A isoform. It contains an
in-frame 96 bp insertion following codon 17 of the isoform GRK4B sequence.
- Biochem. Biophys. Res. Commun. 199: 848-854, 1994

History

Cross references
GenBank X75897

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Sallese M, et al. Two isoforms of G protein-coupled receptor kinase 4 identified by molecular cloning. Biochem. Biophys. Res. Commun. 199: 848-854, 1994. PubMed: 8135832

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