ATCC ATCC Logo 0

IB800

95512

Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
The primers can also be used to generate sequence from the 3' or 5' ends, respectively.

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6; EcoRI--4.6.
Derived from a directionally cloned library with an average insert size of 1.7 kb.
Inserts can be amplified with the M13 universal (forward) and reverse primers. The primers can also be used to generate sequence from the 3' or 5' ends, respectively.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.800000190734863
Intact vector size
3.153
Vector name
lafmid BA
Type of vector
phagemid
Construction
pGEM
Host range
Escherichia coli
Vector end
HindIII; NotI
Vector information
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Cloning sites
HindIII; BamHI; NotI; EcoRI
Insert detection
lacZ', <-, 594-1119
Markers
ampR
MCS
EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
Polylinker sites
HindIII; BamHI; NotI; EcoRI
Primer site
M13 universal, ->; M13 reverse, <-
Promoters
lac
Replicon
f1, ←, 100-556; pMB1, ←, 1518-1518

Insert information

Insert size (kb)
1.5
Type of DNA
cDNA
Insert source
infant brain, 3 months old (from Bento Soares)
Insert tissue
infant brain, 3 months old (from Bento Soares)
Insert information
DESCRIPTION OF INSERT COMPONENT:
Insert 5' end: Modification: HindIII
Insert 3' end: Modification: NotI
Cross references: DNA Seq. Acc.: T03725
Genome
Homo sapiens
Gene name
DNA Segment, expressed
Gene product
DNA Segment, expressed [D0S8933E]
Gene symbol
D0S8933E
Contains complete coding sequence
Unknown

Handling information

Host
Escherichia coli HB101 (ATCC 33694)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI--uncut;
ClaI--4.6; HindIII/NotI-- 3.1, 0.9, 0.6; PvuII--2.8, 1.3, 0.31; ScaI--4.6;
EcoRI--4.6.
- ATCC staff

Derived from a directionally cloned library with an average insert size of 1.7
kb. Inserts can be amplified with the M13 universal (forward) and reverse
primers. The primers can also be used to generate sequence from the 3' or 5'
ends, respectively.
- personal communication

History

Depositors
JM Sikela
Cross references
GenBank 213385
GenBank T03725
GenBank 22753

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Khan AS, et al. Single pass sequencing and physical and genetic mapping of human brain cDNAs. Nat. Genet. 2: 180-185, 1992. PubMed: 1345165

Soares MB, et al. Construction and characterization of a normalized cDNA library. Proc. Natl. Acad. Sci. USA 91: 9228-9232, 1994. PubMed: 7937745

Marcelo B Soares, personal communication

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