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pDRD2E5.6

95511

Detects:
Genome: Homo sapiens
Gene symbol: DRD2
Type of nucleic acid: genomic
Restriction enzyme: TaqI
Number of alleles: 2
Type of polymorphism: site
Alleles:
Allele Freq. Size (kb) Strains
B1 0.13 4.5
B2 0.87 4.0
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0; BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3; HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3.
The insert contains a 4.5Kb TaqI fragment.
The downstream portion of pDRD2E5.6 overlaps the Suarez clone phD2-254.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
8.5600004196167
Intact vector size
2.961
Vector name
pBluescript II SK
Type of vector
phagemid
Host range
Escherichia coli
Vector end
EcoRI
Cloning sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Insert detection
lacZ'
Markers
ampR
Polylinker sites
BssHII; KpnI; ApaI; DraII; XhoI; HincII; AccI; SalI; ClaI; HindIII; EcoRI; PstI; SmaI; BamHI; XbaI; NotI; EagI; SacI
Promoters
T7; T3; lac
Replicon
pMB1; f1

Insert information

Insert size (kb)
5.5999999999999996
Type of DNA
genomic
Insert information
DESCRIPTION OF INSERT COMPONENT:
Genomic copy number: unique
Insert 5' end: EcoRI
Insert 3' end: EcoRI
Cross references:
Genome
Homo sapiens
Chromosome
11
11 q22.2-q22.3
Gene name
dopamine receptor D2
Gene product
dopamine receptor D2 [DRD2]
Gene symbol
DRD2
Alleles
A1, B1, B2, A2
Contains complete coding sequence
Unknown

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 3.0;
BamHI--8.0, 0.6; HindIII--8.6; PstI--4.0, 1.5, 1.35, 0.8, 0.5, 0.3;
HindIII/PstI--3.4, 1.5, 1.3, 0.8, 0.8, 0.5, 0.3.
- ATCC staff

The insert contains a 4.5Kb TaqI fragment. The downstream portion of pDRD2E5.6
overlaps the Suarez clone phD2-254.
- personal communication

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Hauge XY, et al. Detection and characterization of additional DNA polymorphisms in the dopamine D2 receptor gene. Genomics 10: 527-530, 1991. PubMed: 1679742

Suarez BK, et al. Linkage Disequilibria at the D2 dopamine receptor (DRD2) in alcoholics and controls. Genomics 19: 12-20, 1994. PubMed: 7910578

Judith R Kidd, personal communication

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