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Sp3

95505

Detects:
Genome: Homo sapiens
Tissue: Jurkat, Peer, AN4.4, 2052C, 2052D, BJA-B, 300-19, K562,
MEL, HeLa, and NIH 3T3 cell lines
Gene symbol: SP3
Type of nucleic acid: mRNA
Invariant bands (kb): 4.2
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
Mission Collection Item
This is a Mission Collection Item

Documentation

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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
contains sequence Sp3 transcription factor

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BglII--3.8, 0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7.
Insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06; NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.900000095367432
Intact vector size
2.462
Vector name
pSP72
Type of vector
plasmid
Host range
Escherichia coli
Vector end
EcoRI
Vector information
Cross references: DNA Seq. Acc.: X65332
Cloning sites
XhoI; PvuII; HindIII; SphI; PstI; SalI; AccI; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI; ClaI; EcoRV; BglII
Markers
ampR
Polylinker sites
XhoI; PvuII; HindIII; SphI; PstI; SalI; AccI; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI; ClaI; EcoRV; BglII
Promoters
SP6; T7
Replicon
pMB1

Insert information

Insert size (kb)
2.3999999999999999
Type of DNA
cDNA
Insert source
Hut78 cell line, PMA activated
Insert tissue
Hut78 cell line, PMA activated
Insert information
DESCRIPTION OF INSERT COMPONENT:
Cross references: DNA Seq. Acc.: M97191
Nucleotides 1-2221 of the insert correspond to
nucleotides 1-2221 of M97191.
Genome
Homo sapiens
Target gene
Sp3 transcription factor
Gene name
Sp3 transcription factor
Gene product
Sp3 transcription factor [SP3]
Gene symbol
SP3
Contains complete coding sequence
Yes
Insert end
EcoRI

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): BglII--3.8,
0.96; EcoRI--2.5, 2.2; NotI--4.7; PstI--3.0, 0.8, 0.6, 0.3; XbaI--4.7.
- ATCC staff

Insert contains the following restriction sites (approximate kb from the 5'
end): BglII--0.89; EcoRI--0.0, 2.22; EcoRV--2.07; KpnI--1.63; NcoI--1.06;
NotI--0.04; PstI--0.66, 1.43, 1.98; PvuII--1.12; SacI--1.71; XbaI--0.62.
- Mol. Cell. Biol. 12: 4251-4261, 1992

History

Cross references
GenBank M97191

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Kingsley C, Winoto A. Cloning of GT box-binding proteins: a novel Sp1 multigene family regulating T-cell receptor gene expression. Mol. Cell. Biol. 12: 4251-4261, 1992. PubMed: 1341900

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