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D15

95487

Detects:
Genome: Homo sapiens
Tissue: HL-60 cell line; neutrophils; monocytes; eosinophils
Gene symbol: FPR1
Type of nucleic acid: mRNA
Invariant bands (kb): 1.4
Organism
Homo sapiens, human
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1.
The insert includes the following restriction sites (approximate kb from the 5' end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18.
Lacks exon 2 in the 5' untranslated region that is present in some alternatively spliced transcripts. Begins within exon 1 and contains the complete exon 3.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.349999904632568
Intact vector size
2.960
Vector name
pBluescript SK-
Type of vector
phagemid
Construction
pUC19
Host range
Escherichia coli
Vector end
EcoRI; XhoI
Cloning sites
KpnI; ApaI; DraII; XhoI; SalI; HincII; AccI; ClaI; HindIII; EcoRV; EcoRI; PstI; SmaI; BamHI; SpeI; XbaI; EagI; NotI; SacII; BstXI; SacI
Insert detection
lacZ', <-
Markers
ampR
MCS
KpnI...SacI, ->
Polylinker sites
SacI; BstXI; SacII; NotI; EagI; XbaI; SpeI; BamHI; SmaI; PstI; EcoRI; EcoRV; HindIII; (ClaI Bsp106I); (HincII AccI SalI); XhoI; (Eco0109I DraII ApaI); KpnI
Primer site
M13-20, ->; M13 reverse, <-
Promoters
T7; T3; lac
Replicon
f1, →; pMB1

Insert information

Insert size (kb)
1.3500000000000001
Type of DNA
cDNA
Insert source
HL-60 cell line
Insert tissue
HL-60 cell line
Insert information
DESCRIPTION OF INSERT COMPONENT:
Disease: promyelocytic leukemia
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Cross references: DNA Seq. Acc.: L10820
Nucleotides 1-1350 of the insert correspond to
Disease: promyelocytic leukemia
nucleotides 525-552, 5370-6599 of L10820.
Genome
Homo sapiens
Chromosome
19
19 q13.4
Gene name
formyl peptide receptor 1
Gene product
formyl peptide receptor 1 [FPR1]
Gene symbol
FPR1
Contains complete coding sequence
Yes

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3;
XhoI--4.3; EcoRI/XhoI--2.9, 1.4; BamHI--4.3; PstI--3.2, 1.1.
- ATCC staff

The insert includes the following restriction sites (approximate kb from the 5'
end): NcoI--0.25; PvuII--1.0; PstI--1.14; SacI--1.18.
- GenBank/EMBL/DDBJ

Lacks exon 2 in the 5' untranslated region that is present in some
alternatively spliced transcripts. Begins within exon 1 and contains the
complete exon 3.
- Gene 133: 285-290, 1993

History

Cross references
GenBank L10820

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

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Disclaimers

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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Murphy PM, et al. Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133: 285-290, 1993. PubMed: 8224916

Murphy PM, McDermott D. Functional expression of the human formyl peptide receptor in Xenopus oocytes requires a complementary human factor. J. Biol. Chem. 266: 12560-12567, 1991. PubMed: 1712023

Murphy PM, et al. A structural homologue of the N-formyl peptide receptor. Characterization and chromosome mapping of a peptide chemoattractant receptor family. J. Biol. Chem. 267: 7637-7643, 1992. PubMed: 1373134

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