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p4

95485

Detects:
Genome: Homo sapiens
Tissue: HL-60, U937, THP-1 cell lines; B lymphocytes; neutrophils;
monocytes; T lymphocytes
Gene symbol: CMKBR1
Type of nucleic acid: mRNA
Invariant bands (kb): 3.0
Organism
Homo sapiens
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
Shipping information
Rehydrate with TE
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Biosafety Icon BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0, 2.2; EcoRI--4.6, 0.6; XhoI--5.2; EcoRI/XhoI--3.0, 1.6, 0.6; PstI--4.6, 0.6.
The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.45; EcoRI--0.59; PstI--0.61.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.199999809265137
Intact vector size
2.960
Vector name
pBluescript SK-
Type of vector
phagemid
Construction
pUC19
Host range
Escherichia coli
Vector end
EcoRI; XhoI
Cloning sites
KpnI; ApaI; DraII; XhoI; SalI; HincII; AccI; ClaI; HindIII; EcoRV; EcoRI; PstI; SmaI; BamHI; SpeI; XbaI; EagI; NotI; SacII; BstXI; SacI
Insert detection
lacZ', <-
Markers
ampR
MCS
KpnI...SacI, ->
Polylinker sites
SacI; BstXI; SacII; NotI; EagI; XbaI; SpeI; BamHI; SmaI; PstI; EcoRI; EcoRV; HindIII; (ClaI Bsp106I); (HincII AccI SalI); XhoI; (Eco0109I DraII ApaI); KpnI
Primer site
M13-20, ->; M13 reverse, <-
Promoters
T7; T3; lac
Replicon
f1, →; pMB1

Insert information

Insert size (kb)
2.1560000000000001
Type of DNA
cDNA
Insert source
HL-60 cell line
Insert tissue
HL-60 cell line
Insert information
DESCRIPTION OF INSERT COMPONENT:
Disease: promyelocytic leukemia
Insert 5' end: Modification: EcoRI linker
Insert 3' end: Modification: XhoI linker
Cross references: DNA Seq. Acc.: L10918
Nucleotides 1-2156 of the insert correspond to
Disease: promyelocytic leukemia
nucleotides 1-2156 of L10918.
Genome
Homo sapiens
Chromosome
3
3 p21
Gene name
chemokine (C-C) receptor 1
Gene product
chemokine (C-C) receptor 1( chemokine (C-C) receptor 1, RANTES receptor) [CMKBR1]
Gene symbol
CMKBR1
Contains complete coding sequence
Yes

Handling information

Handling notes
Restriction digests of the clone give the following sizes (kb): BamHI/XhoI--3.0,
2.2; EcoRI--4.6, 0.6; XhoI--5.2; EcoRI/XhoI--3.0, 1.6, 0.6; PstI--4.6, 0.6.
- ATCC staff

The insert contains the following restriction sites (approximate kb from the 5'
end): KpnI--0.45; EcoRI--0.59; PstI--0.61.
- GenBank/EMBL/DDBJ

History

Cross references
GenBank L10918

Legal disclaimers

Intended use
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While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Gao JL, et al. Structure and functional expression of the human macrophage inflammatory protein 1alpha/RANTES receptor. J. Exp. Med. 177: 1421-1427, 1993. PubMed: 7683036

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