Pseudomonas fluorescens Migula
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a second tube of broth, a slant, and/or plate.
5. Incubate all tubes and plate at 26°C for 24-48 hours.
Cells examined under wet mount appear as motile rods in singles and chains.
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Huang HT, English AR. Production of 6-aminopenicillanic acid. US Patent 3,239,427 dated Mar 8 1966
Terada O, Uwajima T. Process for the preparation of cholesterol esterase. US Patent 4,011,138 dated Mar 8 1977