Bacillus pumilus Meyer and Gottheil
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 30°C for 24 hours.
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Smith NR, et al. Aerobic spore forming bacteria. US Dep Agric Monogr 16: 1-148, 1952.