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Pseudomonas chlororaphis subsp. chlororaphis (Guignard and Sauvageau) Bergey et al.


Bacterial type strain
Product category
Strain designation
NRRL B-560 [BUCSAV 286, CCEB 292, IFO 3904, NCIB 9392, Stanier 30]
Type strain
Isolation source
Plate contaminant
Product format
Storage conditions
2°C to 8°C
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information


Specific applications
Produces 6-aminopenicillanic acid
Produces carnitine L-carnitine
Produces chlororaphin
Produces lipases active at pH 5.5 and 7.5
Produces provitamin C 2-keto-L-gulonic acid

Handling information

Handling procedure
  1. Open vial according to enclosed instructions or visit for instructions.
  2. Rehydrate the entire pellet with approximately 0.5 mL of #3 broth. Aseptically transfer the entire contents to a 5-6 mL tube of #3 broth. Additional test tubes can be inoculated by transferring 0.5 mL of the primary broth tube to these secondary tubes.
  3. Use several drops of the primary broth tube to inoculate a #3 plate and/or #3 agar slant.
  4. Incubate at 26°C for 24 hours.
Handling notes

This strain is cited to produce 6-aminopenicillanic acid (US Patent 3,239,427), carnitineL-carnitine (US Patent 4,650,759), chlororaphin, lipases active at pH 5.5 and 7.5 (J Ind Microbiol 13: 242-248, 1994), and provitamin C 2-keto-L-gulonic acid (US Patent 3,922,194).

Additional information on this culture is available on the ATCC® web site at


Deposited as
Pseudomonas chlororaphis (Guignard and Sauvageau) Bergey et al.
Chain of custody
ATCC <-- NRRL <-- W. Schmidt
Cross references
GenBank AF094723 Pseudomonas chlororaphis 16S ribosomal RNA gene, partial sequence.
GenBank D86004 Pseudomonas putida 16S ribosomal RNA gene, strain: IFO 3904, partial sequence.
GenBank D86019 Pseudomonas putida gyrB gene for DNA gyrase B subunit, partial cds, strain: IFO 3904.
GenBank D86036 Pseudomonas putida rpoD gene for RNA polymerase sigma-70 factor, partial cds, strain: IFO 3904.

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Huang HT, English AR. Production of 6-aminopenicillanic acid. US Patent 3,239,427 dated Mar 8 1966

Sonoyama T, et al. Process for producing 2-keto-L-gulonic acid. US Patent 3,922,194 dated Nov 25 1975

Yokozeki K, Kubota K. Method of producing L-carnitine. US Patent 4,650,759 dated Mar 17 1987

Liu PV. Survey of hemolysin production among species of pseudomonads. J. Bacteriol. 74: 718-727, 1957. PubMed: 13502298

Bates JL, Liu PV. Complementation of lecithinase activities by closely related pseudomonads: its taxonomic implication. J. Bacteriol. 86: 585-592, 1963. PubMed: 14066442

View All Curated Citations for this Product

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