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Bacillus atrophaeus Nakamura

9372

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Bacillus atrophaeus strain NRS 1221A is a whole-genome sequenced bacterial strain with applications as a test microorganism for sterilization and sterility assurance.
Product category
Bacteria
Strain designation
NRS 1221A
Type strain
No
Genome sequenced strain
Yes
Former name
Bacillus subtilis (Ehrenberg) Cohn
Applications
Bioinformatics
Media testing
Quality control
Pharmaceutical testing
Agricultural research
Product format
Freeze-dried
Storage conditions
2°C to 8°C
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.

Detailed product information

General

Specific applications
Resistance Testing
Dry heat sterilization control
Efficacy testing
Ethylene oxide sterilization control
Media testing
Steam sterilization control
Sterility assurance
Sterilization control
Pharmaceutical and Personal Care
Recommended as a test microorganism for EP 5.1.2 Biological Indicators of Sterilization
Recommended as a test microorganism for JP 16 G4 Microorganisms: Preservatives-Effectiveness Tests
Recommended as a test microorganism for USP <1211> Sterilization and Sterility Assurance of Compendial Articles

Potential plant growth promoting strain; Biofertilizer

Preceptrol
No

Characteristics

Comments
This strain is recommended by ATCC for use in the tests described in military specification MIL S-36586A and U.S. Pharmacopeia, 23rd rev., <1211>, pp. 1976-1981, 1995 where only the taxon is specified.
Variations in colonial morphology and pigmentation occur on nutrient agar; strain is orange on beef extract glucose agar and black on tyrosine agar. Selection of colonies for pigment enhancement is not advised.
Respiration as a function of osmotic pressure
Sterilizing effects of airborne sound waves
Inactivation by oxygen gas plasma

Handling information

Medium
Temperature
30°C
Atmosphere
Aerobic
Handling procedure
  1. Open vial.
  2. Using a single tube of #3 broth (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a Pasteur or 1.0 mL pipette. Rehydrate the entire pellet.
  3. Aseptically transfer this aliquot back into the broth tube. Mix well.
  4. Use several drops of the suspension to inoculate a #3 agar slant and/or plate.
  5. Incubate the tubes and plate at 30°C for 24 hours.
Handling notes
To scale-up the growth of this strain, incubate in a shaking incubator.
Additional information on this culture is available on the ATCC web site at www.atcc.org.

Quality control specifications

Verification method
Whole-genome Sequencing

History

Deposited as
Bacillus subtilis var. niger Smith et al.
Depositors
NR Smith

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Biochim. Biophys. Acta 62: 145, 1962.

Nelson CL, Berger TJ. Inactivation of microorganisms by oxygen gas plasma. Curr. Microbiol. 18: 275-276, 1989.

Nakamura LK. Taxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov.. Int. J. Syst. Bacteriol. 39: 295-300, 1989.

Pisano MA, et al. Sterilizing effects of high-intensity airborne sonic and ultrasonic waves. Appl. Microbiol. 14: 732-736, 1966. PubMed: 4961527

British Pharmacopoeia Commission Methods of sterilization. London, UK:British Pharmacopoeia Commission;British Pharmacopoeia Appendix XVIII, 2003

View All Curated Citations for this Product

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