Acetobacter pasteurianus subsp. pasteurianus (Hansen) Beijerinck and Folpmers
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2. Using a single tube of #1 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #1 agar slant and/or plate. However, it is advisable to establish good growth in the primary broth tube before making subsequent transfers.
5. Incubate all tubes and plate at 26oC. The broth requires 48 to 72 hours to grow. Additional incubation may be required for growth on solid medium.
Colonies on #1 agar are glistening, smooth, translucent and yellowish-gray.
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