Curtobacterium flaccumfaciens (Hedges) Collins and Jones pathovar poinsettiae
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2. Using a single tube of #44 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #44 agar slant and/or plate.
5. Incubate the tubes and plate at 30°C for 24 hours.
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Collins MD, Jones D. Reclassification of Corynebacterium flaccumfaciens, Corynebacterium betae, Corynebacterium oortii and Corynebacterium poinsettiae in the genus Curtobacterium, as Curtobacterium flaccumfaciens comb. nov.. J. Gen. Microbiol. 129: 3545-3548, 1983.