Azotobacter chroococcum Beijerinck
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2. From a single tube of #11 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette and use to rehydrate the entire pellet.
3. Aseptically transfer the rehydrated pellet back into the broth tube. Mix well.
4. Incubate at 26oC. Initial growth should occur in 3 to 5 days.
5. After growth appears in the broth, a second tube of broth, a slant, and/or a plate may be inoculated. It is advisable to establish good growth in the primary tube before making subsequent transfers.
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