Acinetobacter johnsonii Bouvet and Grimont
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2. Using a single tube of #3 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate an additional broth tube, a #3 agar slant and/or a plate.
5. Incubate all tubes and plate at 26°C for 24 hours.
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Mitsugi K, et al. Process for preparing 5'-nucleotides. US Patent 3,296,087 dated Jan 3 1967
Yokozeki K, Kubota K. Method of producing L-carnitine. US Patent 4,650,759 dated Mar 17 1987
Baumann P, et al. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J. Bacteriol. 95: 1520-1541, 1968. PubMed: 5650064