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pTSEC

87792

This vector overproduces yeast truncated cystathionine beta-synthase (CBS).   The truncated version was made by using PCR to insert two nonsense codons (TAA and TAG) after the site encoding the residue 353 amino acid of the full length CBS (F-CBS) gene on pF-SEC (ATCC# 87791).                                      ?Biochemistry 39: 10548-10556, 2000
Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Shipping information
Escherichia coli containing the plasmid in glycerol stock
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
cloning vector

Characteristics

Genotype
E. coli CB149 genotype: delta trp EDCBA2 hsdR514 (rk- mk+) supE44 supF58 lacY1 lacU169 galK2 galT22.
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--uncut; EcoRI--5.6; PstI--5.6; EcoRI/PstI--4.4, 1.3.
Overproduces yeast cystathionine beta-synthase (truncated).
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
5.679999828338623
Vector name
pTSEC (plasmid)
Construction
pSTR4-2
Vector information
primer site: 5'-TTGACAATTAATCATCCGGC-3'
Markers
ampR
Primer site
5'-TTGACAATTAATCATCCGGC-3'
Promoters
Ptrc
Terminator
rrnBT1; rrnBT2

Insert information

Gene product
cystathionine beta synthase

Handling information

History

Deposited as
Saccharomyces cerevisiae Hansen, teleomorph
Depositors
EW Miles
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

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References

Curated Citations

Jhee KH, et al. Domain architecture of the heme-independent yeast cystathionine beta-synthase provides insights into mechanisms of catalysis and regulation. Biochemistry 39: 10548-10556, 2000. PubMed: 10956046

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