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SRS for Detecting Protein-Protein Interactions

87639

Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

Characteristics

Mycoplasma contamination
Not detected

Vector information

Intact vector size
6.828
Vector name
pMS-TRP
Type of vector
phagemid
Host range
Saccharomyces cerevisiae
Escherichia coli
Vector end
EcoRI; XhoI
Vector information
Other unique sites: HpaI SwaI mLuI NotI BglI MunI BglII
other: Myristoylation site, ->
epitope tag: 3x hemaglutinin (HA), ->
other: lox site
other: lox site
Cross references:
Cloning sites
EcoRI; SacI; KpnI; XhoI; SphI
Markers
ampR; TRP1
MCS
EcoRI...SphI, ->
Polylinker sites
EcoRI; SacI; KpnI; XhoI; SphI
Replicon
2 micron ori; f1; pMB1
Restriction sites
NotI
Terminator
CYC1, ->

Insert information

Insert size (kb)
0.296
Type of DNA
cDNA
Insert information
Insert 5' end: EcoRI
Insert 3' end: XhoI
Cross references: DNA Seq. Acc.: V01512
Genome
human
Chromosome
14
14q 24.3
Gene name
FOS
Gene symbol
FOS
Contains complete coding sequence
No

Handling information

Host
Escherichia coli JM107 (ATCC 47014)
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI/XhoI--6.8,
0.3; HindIII--4.3, 2.8; XbaI--3.3, 3.3, 0.4, 0.2.
- ATCC staff

Positive control for SOS Recruitment System. The insert contains the leucine
zipper motif.
- personal communication

SOS recruitment system (SRS) is a genetic screening method to detect proteins
interacting in the cytoplasm. It is based on membrane targeting with a
myrisoylation signal and SOS-based activation of Ras protein.
- Mol. Cell. Biol. 17: 3094-3102, 1997

History

Depositors
SJ Elledge
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Aronheim A, et al. Isolation of an AP-1 repressor by a novel method for detecting protein-protein interactions. Mol. Cell. Biol. 17: 3094-3102, 1997. PubMed: 9154808

Brachmann RK, Boeke JD. Tag games in yeast: the two-hybrid system and beyond. Curr. Opin. Biotechnol 8: 561-568, 1997. PubMed: 9353226

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