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pDS472a

87615

Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried

Documentation

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Detailed product information

General

Specific applications
encodes removable tag for protein isolation
expression vector

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): HindIII--6.2, 1.9, 1.1; EcoRI--8.0, 1.2; PstI--9.2.
The pDS472 5' oligo changed the ATG of GST to TTG (M1L) in order to prevent translation of only the GST portion of a fusion protein.
The vector was designed for C-terminal tagging with GST (glutathione S transferase). The vector contains a thrombin cleavage site (LVPR/GS) immediately following the GST moiety, to allow removal of the tag following purification.
The vector was constructed by 1) amplification of the Schistosoma japonicum glutathione S-transferase gene with primers designed to flank the gene and modify the polylinker, 2) gel purification and digest of the PCR product with XhoI,
3) ligation into pREP4X cleaved with XhoI and SmaI; the SmaI site was lost during cloning, 4) digestion with NotI & SalI, purified GST-containing fragment was cloned into pSLF172, digested with NotI and SalI.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
9.199999809265137
Vector name
pDS472a (phagemid)
Type of vector
phagemid
Construction
REP4X
Vector information
tag (C-terminal): bacterial GST
Markers
ampR; ura4+
MCS
XhoI...SmaI
Promoters
Expression: nmt1 (full strength)
Replicon
ars1; f1; pMB1

Handling information

History

Depositors
SL Forsburg
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

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Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Forsburg SL, Sherman DA. General purpose tagging vectors for fission yeast. Gene 191: 191-195, 1997. PubMed: 9218719

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