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pRat alpha 2

87405

Detects:
Genome: rat
Tissue: kidney, brain, heart, adipose, muscle, stomach, and lung
Gene symbol: ATP1A2
Type of nucleic acid: mRNA
Invariant bands (kb): 3.4, 5.3
Organism
Rattus norvegicus, rat
Clone type
Clone
Applications
Molecular biology
Product format
Freeze-dried
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
isoform-specific probe ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide

Characteristics

Comments
This is a 5' specific probe for the alpha2 isolform. It is not full length. Nucleotides 1-381 of the insert correspond to nucleotides 121-502 of Genbank M14512.
The insert contains the following restricti sites (approximate kb from the 5' end): SacI--0.06, 0.17; NcoI--0.09, 0.33.
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7, 0.4; BglI--2.0, 1.2; PvuII--2.4, 0.7.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
3.16700005531311
Intact vector size
2.686
Vector name
pUC18
Type of vector
plasmid
Construction
pUC71K
Host range
Escherichia coli
Vector end
SmaI
Vector information
Cross references: DNA Seq. Acc.: L08752
Cloning sites
HindIII; SphI; PstI; SalI; AccI; HincII; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI
Insert detection
lacZ', <-
Markers
ampR
MCS
HindIII...EcoRI, ->
Polylinker sites
HindIII; SphI; PstI; SalI; AccI; HincII; XbaI; BamHI; SmaI; KpnI; SacI; EcoRI
Promoters
lac
Replicon
pMB1, <-

Insert information

Insert size (kb)
0.38100000000000001
Type of DNA
cDNA
Insert source
brain
Insert strain
CD
Insert tissue
brain
Insert information
DESCRIPTION OF INSERT COMPONENT:
Insert 5' end: ScaI
Insert 3' end: NheI Modification: blunted
Cross references: DNA Seq. Acc.: M14512
Nucleotides 1-381 of the insert correspond to
nucleotides 121-502 of M14512.
Genome
rat
Gene name
ATPase, Na+/K+ transporting, alpha 2 polypeptide
Gene product
ATPase, Na+/K+ transporting, alpha 2 (+) polypeptide [ATP1A2]
Gene symbol
ATP1A2
Contains complete coding sequence
No

Handling information

Host
Escherichia coli DH5alpha
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI/PstI--2.7,
0.4; BglI--2.0, 1.2; PvuII--2.4, 0.7.
- ATCC staff

The insert contains the following restricti sites (approximate kb from the 5'
end): SacI--0.06, 0.17; NcoI--0.09, 0.33.
- GenBank/EMBL/DDBJ

History

Depositors
JB Lingrel
Special collection
NCRR Contract
Cross references
GenBank M14512

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Young RM, Lingrel JB. Tissue distribution of mRNAs encoding the alpha isoforms and beta subunit of Rat Na+, K+-ATPase. Biochem. Biophys. Res. Commun. 145: 52-58, 1987. PubMed: 3036133

Orlowski J, Lingrel JB. Tissue-specific and developmental regulation of Rat Na,K-ATPase catalytic alpha isoform and beta subunit mRNAs. J. Biol. Chem. 263: 10436-10442, 1988. PubMed: 2839491

Shull GE, et al. Molecular cloning of three distinct forms of the Na+,K+-ATPase alpha subunit from Rat brain. Biochemistry 25: 8125-8132, 1986. PubMed: 3028470

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