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lambdaYES-R

87271

Clone type
Vector
Applications
Molecular biology
Product format
Frozen
Shipping information
bacteria-free lysate
Mission Collection Item
This is a Mission Collection Item.

Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
expression vector
lambda vector allowing conversion to plasmid
vector for constructing cDNA libraries

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0, 20.0; NotI--44.0; XhoI--24.0, 20.0.
The plasmid derivative (pSE936, 7.8 kb) is a YC-type shuttle expression vector.
Transcription initiated from the GAL1 promoter in yeast hosts can be terminated at the HIS3 terminators. GAL1 does not provide translational start signals.
Vector for the construction of cDNA libraries permitting regulated expression in both yeast and E. coli and simple conversion to plasmid via the cre-lox system.
The lac promoter contains a ribosome binding sequence optimally spaced from an AUG start codon and can support translational fusions.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
42.6
Intact vector size
42.600
Vector name
lambdaYES-R (phage, lambda - replacement)
Type of vector
phage
Construction
lambdagt6; pSE936
Host range
Escherichia coli
Vector information
other: left arm (19.6 kb), ->
other: lox, ->
other: lox, ->
other: right arm (15.2 kb, with cI857)
other: left arm (19.6 kb)
other: lox
other: right arm (15.2 kb, with cI857)
Centromere
CEN4
Cloning sites
XhoI; EcoRI
Markers
ampR; URA3
MCS
XhoI EcoRI, ->
Polylinker sites
EcoRI; XhoI; EcoRI
Promoters
GAL1, ->; lac, <-
Replicon
ARS1; pMB1
Terminator
HIS3, ->
Transcription terminator
HIS3

Handling information

Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI--24.0,
20.0; NotI--44.0; XhoI--24.0, 20.0.
- ATCC staff

Vector for the construction of cDNA libraries permitting regulated expression in
both yeast and E. coli and simple conversion to plasmid via the cre-lox system.
The plasmid derivative (pSE936, 7.8 kb) is a YC-type shuttle expression vector.
- personal communication

The lac promoter contains a ribosome binding sequence optimally spaced from an
AUG start codon and can support translational fusions.
- Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991

Transcription initiated from the GAL1 promoter in yeast hosts can be terminated
at the HIS3 terminators. GAL1 does not provide translational start signals.
- Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991

History

Depositors
SJ Elledge
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Stephen J Elledge, personal communication

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