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pUCP19 in Escherichia coli DH5alphaF’


Clone type
Molecular biology
Product format
Shipping information
Escherichia coli DH5αF containing the plasmid
Storage conditions
-80°C or colder
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
Expression vector
Shuttle vector
Vector containing primer sites useful for sequencing
Vector permitting construction of fusion proteins
Vector permitting visual detection of recombinants


Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--4.5; EcoRI--4.5; HindIII--4.5; EcoRI/PvuII--3.7, 0.47, 0.31, 0.16; HincII--3.9, 0.6; PvuI--2.7, 1.8.

Plasmid is maintained in P. aeruginosa at 10-25 copies per cell.

Vector allows screening of recombinants on LB XGal indicator medium as well as on MacConkey lactose medium.

Shuttle expression vector allowing visual detection of recombinant molecules in a broad host range.

Constructed by insertion of a 1.8 kb PstI fragment from pRO1614 into the NarI site of pUC19.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
Vector name
pUCP19 (plasmid)
Type of vector
pUC19, pRO1614
Coding sequence
CbR; ampR
Expression: lac
bhr (rep pRO1600); pMB1

Handling information

Pseudomonas aeruginosa PAO1-LAC (ATCC 47085); Pseudomonas aeruginosa PA103-LAC (ATCC 47086)
Host range:
Broad host range; Pseudomonas aeruginosa; Escherichia coli

Host requirements:
E. coli lacIq
E. coli lacZdeltaM15 for recombinant screening
P. aeruginosa lacIq
P. aeruginosa lacZdeltaM15 for recombinant screening

Suggested hosts:
Pseudomonas aeruginosa PAO1-LAC (ATCC 47085)
Pseudomonas aeruginosa PA103-LAC (ATCC 47086)
Handling procedure
Aseptically add 0.3 to 0.4 mL of liquid medium to the freeze-dried pellet and mix well.  Transfer 100 µL to a test tube containing 5 mL LB+50 µg/mL of ampicillin. A loopful of culture can also be streaked on an LB + amp agar plate. Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures.                   


HP Schweizer, University of Calgary
Special collection
NCRR Contract

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.


This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.



Curated Citations

Karkhoff-Schweizer RR, Schweizer HP. Utilization of a mini-Dlac transposable element to create an alpha-complementation and regulated expression system for cloning in Pseudomonas aeruginosa. Gene 140: 7-15, 1994. PubMed: 8125342

Schweizer HP. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 97: 109-112, 1991. PubMed: 1899844

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