pASK84

87094 ^™

This is an immunoglobulin expression vector allowing cloning of variable region domains and efficient purification of Fab fragments via immobilized metal affinity chromatography. It contains the mouse immunoglobulin heavy chain region, part of the hinge region and the mouse immunoglobulin kappa constant region. The heavy chain and light chain variable regions may be cloned into the PstI-EcoRI-BstEII and SstI-PmI-XhoI multiple cloning sites respectively. The other unique restriction sites (XbaI NcoI HindIII) may be used for subcloning or exchange of immunoglobulin constant regions. Grow at 22⁰ C for expression.

Organism: Mus musculus, mouse
Classification: Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
Clone type: Vector
Applications: Molecular biology
Product format: Freeze-dried
Shipping information: Escherichia coli containing the plasmid
Storage conditions: 2°C to 8°C

See Additional Product Information

Price: $639.00 EA

Discounts may be available for our fellow nonprofit organizations. Login to see your price.

Generally ships within 1-3 business days

Select specific lot(s)

Please enter the quantity for the selected lot number and add to cart.

Please fill at least one quantity field.

Quantity should not be greater than inventory.

Quantity	Lot Number	Inventory	Donor Age	Donor Sex	Donor Ethnicity	Donor BMI	CoA

Documentation

Product sheet

BSL 1

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

General

Specific applications: expression vector
vector permitting construction of fusion proteins
vector with sequences for protein export

Characteristics

Comments: Restriction digests of the clone give the following sizes (kb): NcoI/XbaI--4.0, 0.5; HindIII/NcoI--4.0, 0.5; EcoRI--4.6.
Contains part of the hinge region of immunoglobulin gamma 1.
The heavy chain and light chain variable regions may be cloned into the PstI-EcoRI-BstEII and SstI-PmlI-XhoI multiple cloning sites respectively.
The other unique restriction sites (XbaI NcoI HindIII) may be used for subcloning or exchange of immunoglobulin constant regions.
Immunoglobulin expression vector allowing cloning of variable region domains and efficient purification of Fab fragments via immobilized metal affinity chromatography.
Contains the immunoglobulin heavy chain region, part of the hinge region and the immunoglobulin kappa constant region.
The products are secreted into the periplasm where protein folding and disulfide bond formation occurs.
Mycoplasma contamination: Not detected

Vector information

Construct size (kb): 4.586999893188477
Vector name: pASK84 (plasmid)
Type of vector: plasmid
Construction: pASK30, pASK37-IgG1
Vector information: leader peptide: phoA
signal peptide coding region: ompA
epitope tag: hexahistidine sequence (6-His)
Coding sequence: Ig gamma 1 constant region; Ig kappa constant region
Markers: ampR
Promoters: Expression: lacUV5
Replicon: f1; pMB1
Repressor gene: lacI
Restriction sites: HindIII; NcoI; XbaI
Terminator: lpp

Insert information

Insert size (kb): 0.31
Type of DNA: genomic
Gene product: immunoglobulin gamma 1 (Gm marker)(immunoglobulin heavy chain constant region, gamma 1) [IgkC]

Handling information

Medium: ATCC Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Temperature: 37°C
Handling notes: Restriction digests of the clone gave the following sizes (in kb): NcoI/XbaI 4.0, 0.5 ; HindIII/NcoI 4.0, 0.5 ; EcoRI 4.6. ATCC Staff

History

Depositors: A Skerra
Special collection: NCRR Contract
Cross references: GenBank X84995

Legal disclaimers

Intended use

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

Warranty

The product is provided 'AS IS' and the viability of ATCC^® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

References

Curated Citations

Skerra A. A general vector, pASK84, for cloning, bacterial production, and single-step purification of antibody Fab fragments. Gene 141: 79-84, 1994. PubMed: 8163179

Arne Skerra, personal communication

For product-related inquiries and issues, contact Product Experience:

Message Us

Send us a message

Hours of Operation

Monday - Friday

9:00am - 5:00pm
US Eastern Time