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pHSG-cre

87075

Organism
Staphylococcus aureus subsp.aureus bacteriophage P1
Clone type
Vector
Applications
Molecular biology
Product format
Frozen
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Documentation

ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
produces protein recombinase, site-specific
vector for other uses

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--2.6, 1.4; EcoRI--4.0; PstI--2.6, 1.4.
The cre gene contains the following restriction sites (approximate kb from the 5' end): BamHI--0.84; ClaI--1.29.
The lambda/plasmid hybrid vectors can then be used for automatic subcloning; that is, the cre-mediated excision of plasmid constructs.
A plasmid useful for generating novel lambda/plasmid hybrid vectors by using cre-lox recombination to exchange new plasmids for plasmids already in lambda/plasmid hybrids.
Constructed by ligating a 1.3 kb cre-containing fragment from lambdaKC to pHSG298 digested with EcoRI+SalI.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
4.0
Intact vector size
2.676
Vector name
pHSG298
Type of vector
plasmid
Construction
pHSG1339; pHSG298, lambdaKC
Host range
Escherichia coli
Vector end
EcoRI; SalI
Vector information
Other unique sites: ClaI
other: cre
Cloning sites
EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; SalI; PstI; SphI
Insert detection
lacZ', <-
Markers
kanR
MCS
HindIII...EcoRI, ->
Polylinker sites
EcoRI; SacI; KpnI; SmaI; BamHI; XbaI; SalI; PstI; SphI; HindIII
Promoters
lac
Replicon
pSC101; pMB1

Insert information

Insert size (kb)
1.3
Type of DNA
genomic
Insert information
Insert 5' end: EcoRI
Insert 3' end: SalI
Cross references: DNA Seq. Acc.: X03453
Genome
bacteriophage P1
Target gene
recombinase, site-specific
Gene name
recombinase, site-specific
Gene product
recombinase, site-specific [cre]
Gene symbol
cre
Contains complete coding sequence
Yes

Handling information

Host
Escherichia coli JM109 (ATCC 53323)
Medium
Temperature
37°C
Handling notes
Restriction digests of the clone give the following sizes (kb): EcoRI/SalI--2.6,
1.4; EcoRI--4.0; PstI--2.6, 1.4.
- ATCC staff

The cre gene contains the following restriction sites (approximate kb from the
5' end): BamHI--0.84; ClaI--1.29.
- GenBank/EMBL/DDBJ

Constructed by ligating a 1.3 kb cre-containing fragment from lambdaKC to
pHSG298 digested with EcoRI+SalI.
- Biotechniques 16: 1060-1064, 1994

A plasmid useful for generating novel lambda/plasmid hybrid vectors by using
cre-lox recombination to exchange new plasmids for plasmids already in
lambda/plasmid hybrids.
- Biotechniques 16: 1060-1064, 1994

The lambda/plasmid hybrid vectors can then be used for automatic subcloning;
that is, the cre-mediated excision of plasmid constructs.
- Biotechniques 16: 1060-1064, 1994

History

Depositors
ML Pall
Special collection
NCRR Contract
Cross references
GenBank X03453

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

Permits & Restrictions

Import Permit for the State of Hawaii

If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.

MORE INFORMATION ABOUT PERMITS AND RESTRICTIONS

Frequently Asked Questions

References

Curated Citations

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Brunelli JP, Pall ML. Lambda/Plasmid vector construction by in vivo cre/lox-mediated recombination. BioTechniques 16: 1060-1064, 1994. PubMed: 8074871

Takeshita S, et al. High-copy-number and low-copy-number plasmid vectors for lacZalpha-complementation and chloramphenicol- or kanamycin-resistance selection. Gene 61: 63-74, 1987. PubMed: 3327753

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