Clone type
Molecular biology
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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information


Specific applications
vector permitting construction of fusion proteins


Restriction digests of the clone give the following sizes (kb): EcoRI--1.3; HindIII--1.3; BglII--1.3.
The vector contains the following restriction sites (approximate kb from nt 1): BglII--0.79; ClaI--0.11; EcoRI--0.33; HindIII--0.02; NcoI--0.12; PstI--0.75; PvuII--0.23.
Replication depends on read-through from the tac promoter. Replication may be negatively affected by cloning of large inserts or sequences containing a transcriptional terminator.
Expression vector permitting production of heterologous peptides fused to active chloramphenicol acetyltransferase.
Repression of this high copy number plasmid is hard to achieve, even using hosts overproducing lacIq. Escherichia coli WK6 is recommended for inducible expression.
The replicon is derived from ColE1 and does not contain the RNAII promoter nor most of the antisense RNAI control element coding sequence. This allows compatibility with other ColE1 plasmids.
The order of the major features of the plasmid is: HindIII - tac promoter - NcoI - cat/ScaI/MCS/BglII - HyRep2 ori.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
Vector name
pJRtac99 (plasmid)
Type of vector
pGV451, tac, Tn9 cat

Handling information


J Robben, G Volckaert
Cross references
GenBank X69551

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.

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This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

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Curated Citations

Robben J, et al. An Escherichia coli plasmid vector system for high-level production and purification of heterologous peptides fused to active chloramphenicol acetyltransferase. Gene 126: 109-113, 1993. PubMed: 7682530

Dekeyzer N, et al. Cloning, expression and purification of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor via a fusion product with chloramphenicol acetyltransferase. Protein Eng. 7: 125-130, 1994. PubMed: 8140089

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