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pJRtac99

87018

Clone type
Vector
Applications
Molecular biology
Product format
Freeze-dried

Documentation

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ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.

Detailed product information

General

Specific applications
vector permitting construction of fusion proteins

Characteristics

Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--1.3; HindIII--1.3; BglII--1.3.
The vector contains the following restriction sites (approximate kb from nt 1): BglII--0.79; ClaI--0.11; EcoRI--0.33; HindIII--0.02; NcoI--0.12; PstI--0.75; PvuII--0.23.
Replication depends on read-through from the tac promoter. Replication may be negatively affected by cloning of large inserts or sequences containing a transcriptional terminator.
Expression vector permitting production of heterologous peptides fused to active chloramphenicol acetyltransferase.
Repression of this high copy number plasmid is hard to achieve, even using hosts overproducing lacIq. Escherichia coli WK6 is recommended for inducible expression.
The replicon is derived from ColE1 and does not contain the RNAII promoter nor most of the antisense RNAI control element coding sequence. This allows compatibility with other ColE1 plasmids.
The order of the major features of the plasmid is: HindIII - tac promoter - NcoI - cat/ScaI/MCS/BglII - HyRep2 ori.
Mycoplasma contamination
Not detected

Vector information

Construct size (kb)
1.392000079154968
Vector name
pJRtac99 (plasmid)
Type of vector
plasmid
Construction
pGV451, tac, Tn9 cat
Markers
cmlR
Promoters
tac
Replicon
HyRep2

Handling information

History

Depositors
J Robben, G Volckaert
Cross references
GenBank X69551

Legal disclaimers

Intended use
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
Warranty

The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid.  Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.

Disclaimers

This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.

While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.

This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.

Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.

References

Curated Citations

Robben J, et al. An Escherichia coli plasmid vector system for high-level production and purification of heterologous peptides fused to active chloramphenicol acetyltransferase. Gene 126: 109-113, 1993. PubMed: 7682530

Dekeyzer N, et al. Cloning, expression and purification of a sarcoplasmic calcium-binding protein from the sandworm Nereis diversicolor via a fusion product with chloramphenicol acetyltransferase. Protein Eng. 7: 125-130, 1994. PubMed: 8140089

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