Lactobacillus acidophilus (Moro) Hansen and Mocquot
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ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
2. Using a single tube of #416 broth (5 to 6 ml), withdraw approximately 0.5 to 1.0 ml with a Pasteur or 1.0 ml pipette. Rehydrate the entire pellet.
3. Aseptically transfer this aliquot back into the broth tube. Mix well.
4. Use several drops of the suspension to inoculate a #416 agar slant and/or plate.
5. Incubate the tubes and plate at 37°C in an atmosphere of 5% CO2 for 24 to 48 hours.
Broth may be turbid at 24 hours. Because of the rapid growth in broth, it is advisable to transfer to fresh media at 24 hours to maintain viability. Growth is best in broth or biphasic slants.
Additional information on this culture is available on the ATCC® web site at www.atcc.org.
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
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Davis GH. The classification of Lactobacilli from the human mouth. J. Gen. Microbiol. 13: 481-493, 1955. PubMed: 13278499
J. Appl. Bacteriol. 18: 274, 1955.
Efthymiou C, Hansen PA. An antigenic analysis of Lactobacillus acidophilus. J. Infect. Dis. 110: 258-267, 1962. PubMed: 13889379
. . Zentralbl. Bakteriol. II Abt. 111: 250, 1958.